iFluor™ 594 Conjugated S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02]
Catalog# HA720125F
iFluor™ 594 Conjugated S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02]
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IF-Tissue
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Human
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iFluor™ 594
Overview
Product Name
iFluor™ 594 Conjugated S100 beta Recombinant Rabbit Monoclonal Antibody [SC57-02]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within C-terminal human S100 beta.
Species Reactivity
Human
Validated Applications
IF-Tissue
Molecular Weight
11 kDa
Positive Control
human malignant melanoma tissue, human meningioma tissue.
Conjugation
iFluor™ 594
Clone Number
SC57-02
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:50-1:200
Target
Function
S100 calcium-binding protein B (S100B) is a protein of the S100 protein family. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100B is glial-specific and is expressed primarily by astrocytes, but not all astrocytes express S100B. It has been shown that S100B is only expressed by a subtype of mature astrocytes that ensheath blood vessels and by NG2-expressing cells. This protein may function in neurite extension, proliferation of melanoma cells, stimulation of Ca2+ fluxes, inhibition of PKC-mediated phosphorylation, astrocytosis and axonal proliferation, and inhibition of microtubule assembly. In the developing CNS it acts as a neurotrophic factor and neuronal survival protein. In the adult organism it is usually elevated due to nervous system damage, which makes it a potential clinical marker.
Background References
1. Duan K et al. S100-beta aggravates spinal cord injury via activation of M1 macrophage phenotype. J Musculoskelet Neuronal Interact. 2021 Sep
2. Hanin A et al. Neuron Specific Enolase, S100-beta protein and progranulin as diagnostic biomarkers of status epilepticus. J Neurol. 2022 Jul
Subcellular Location
Cytoplasm, Nucleus, Secreted.
Synonyms
NEF antibody
Protein S100 B antibody
Protein S100-B antibody
S 100 calcium binding protein beta chain antibody
S 100 protein beta chain antibody
S-100 protein beta chain antibody
S-100 protein subunit beta antibody
S100 antibody
S100 calcium binding protein beta (neural) antibody
S100 calcium-binding protein B antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded human malignant melanoma tissue labeling S100 beta.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720125F S100 beta (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain. -
Immunofluorescence analysis of paraffin-embedded human meningioma tissue labeling S100 beta.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. The section was then incubated overnight at +4℃ with HA720125F S100 beta (iFluor™ 594, red) at 1/100 dilution, washed with PBS. DAPI was used as nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"