iFluor™ 488 Conjugated Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]
Catalog# HA720129F
iFluor™ 488 Conjugated Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]
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IF-Tissue
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IF-Cell
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Human
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated Cytokeratin 5 Recombinant Rabbit Monoclonal Antibody [SC62-04]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human Cytokeratin 5 aa 70-110.
Species Reactivity
Human
Validated Applications
IF-Tissue, IF-Cell
Molecular Weight
Predicted band size: 62 kDa
Positive Control
Human skin tissue, A431.
Conjugation
iFluor™ 488
Clone Number
SC62-04
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:100-1:200
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IF-Cell
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1:100
Target
Function
Cytokeratin 5 is commonly found in the cells on the outermost layer of skin in humans and animals. It is encoded by the KRT5 gene and pairs with the type I keratin K14. Cytokeratin 5 has become an important biomarker for different types of cancer, including mesothelioma, breast cancer and lung cancer. It helps differentiate squamous carcinomas from adenocarcinomas. Pathologists use cytokeratin 5 to distinguish mesothelioma from adenocarcinoma, the most common type of lung cancer. Cytokeratin 5/6 cannot identify mesothelioma on its own. Cytokeratin 5/6 is a positive marker for malignant pleural mesothelioma, found in more than three-fourths of cases. It is also found in certain types of lung cancers and breast cancers. Pathologists use cytokeratin 5/6 to stain cancer tissue samples. Cytokeratin 5/6 immunoreactivity is rarely seen in adenocarcinomas of the lung. If a tumor sample shows strong expression of cytokeratin 5/6, it gives pathologists a hint the tumor is malignant mesothelioma rather than a metastatic adenocarcinoma. However, this marker is not effective for all cell types of mesothelioma. Cytokeratin 5/6 staining is usually weak or negative for sarcomatoid mesothelioma, the least common and hardest-to-treat cell type of the asbestos-related cancer. The marker is also not effective in telling the difference between pleural mesothelioma and squamous cell carcinomas. About 25 to 30 percent of all lung cancers are squamous cell carcinomas.
Background References
1. Terada K et al. Clinicopathological features of cytokeratin 5-positive pulmonary adenocarcinoma. Histopathology. 2023 Feb
2. Li A et al. Altered cytokeratin 5 expression in breast lobular myoepithelial cells. J Clin Pathol. 2024 Jul
Subcellular Location
Cytoplasm.
UNIPROT
Synonyms
58 kDa cytokeratin antibody
CK-5 antibody
CK5 antibody
Cytokeratin-5 antibody
Cytokeratin5 antibody
DDD antibody
DDD1 antibody
EBS2 antibody
epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types antibody
K2C5_HUMAN antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (HA720129F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720129F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. -
Immunocytochemistry analysis of A431 cells labeling Cytokeratin 5 with Rabbit anti-Cytokeratin 5 antibody (HA720129F) at 1/100 dilution.
Cells were fixed in 100% methanol for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 5 antibody (HA720129F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/800 dilution. -
Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 5 (HA720129F), Cytokeratin 1 (HA720141F) and Cytokeratin 10 (HA720119F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 5 (HA720129F, green) at 1/100 dilution, Cytokeratin 1 (HA720141F, red) at 1/400 dilution and Cytokeratin 10 (HA720119F, yellow) at 1/400 dilution overnight at 4 ℃, washed with PBS.
DAPI was used as nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"