iFluor™ 488 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
Catalog# HA720134F
iFluor™ 488 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
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IF-Tissue
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Human
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Rat
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584.
Species Reactivity
Human, Rat
Validated Applications
IF-Tissue
Molecular Weight
Predicted band size: 59 kDa
Positive Control
Human skin tissue, rat skin tissue.
Conjugation
iFluor™ 488
Clone Number
ST05-43
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:400-1:500
Target
Function
Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 10 is a heterotetramer of two type I and two type II keratins, is generally associated with keratin 1, and is seen in all suprabasal cell layers including stratum corneum.
Background References
1. Tabuchi Y. et al. Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene. Exp Anim 63:31-44 (2014).
2. Zhu XJ. et al. BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin. PLoS Genet 10:e1004687 (2014).
Subcellular Location
Cytoplasm.
Synonyms
BCIE antibody
BIE antibody
CK 10 antibody
CK10 antibody
CK-10 antibody
Cytokeratin-10 antibody
EHK antibody
K10 antibody
K1C10_HUMAN antibody
Keratin 10 antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720134F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720134F, green) at 1/500 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. -
Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (HA720134F), Cytokeratin 14 (HA720147F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720134F, green) at 1/400 dilution, Cytokeratin 14 (HA720147F, red) at 1/400 dilution and Vimentin (EM0401, yellow) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"