iFluor™ 488 Conjugated Cytokeratin 17 Recombinant Rabbit Monoclonal Antibody [SR45-06]
Catalog# HA720138F
iFluor™ 488 Conjugated Cytokeratin 17 Recombinant Rabbit Monoclonal Antibody [SR45-06]
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IF-Tissue
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IF-Cell
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Human
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Mouse
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated Cytokeratin 17 Recombinant Rabbit Monoclonal Antibody [SR45-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Cytokeratin 17 aa 1-50 / 432.
Species Reactivity
Human, Mouse
Validated Applications
IF-Tissue, IF-Cell
Molecular Weight
Predicted band size: 48 kDa
Positive Control
Human esophagus tissue, Hela.
Conjugation
iFluor™ 488
Clone Number
SR45-06
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:200
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IF-Cell
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1:100
Target
Function
Cytokeratin 17 is a member of the Cytokeratin subfamily of intermediate filament proteins (IFPs). It is unique in that it is normally expressed in the basal cells of complex epithelia but not in stratified or simple epithelia. Cytokeratin 17 contains 432 amino acids and is expressed in the nail bed, hair follicle, sebaceous glands and other epidermal appendages. Cytokeratin 17 functions to regulate cell growth and size through its interactions with the adaptor protein 14-3-3-sigma to mediate protein synthesis. Mutations in the gene encoding for Cytokeratin 17 lead to depressed protein translation and smaller sized skin keratinocytes, corresponding to decreased Akt/mTOR signaling activity. Cytokeratin 17 may be a useful marker for cervical stem cell identification, squamous cell carcinoma of the larynx, respiratory syncytial virus and transitional cell carcinomas of the human urinary tract.
Background References
1. Doucet YS. et. al. The touch dome defines an epidermal niche specialized for mechanosensory signaling. Cell Rep 3:1759-65 (2013).
2. Johnson EK. et. al. Identification of new dystroglycan complexes in skeletal muscle. PLoS One 8:e73224 (2013).
Subcellular Location
Cytoplasm.
Synonyms
39.1 antibody
CK 17 antibody
CK17 antibody
CK-17 antibody
Cytokeratin-17 antibody
K17 antibody
K1C17_HUMAN antibody
Keratin 17 antibody
keratin 17 epitope S1 antibody
keratin 17 epitope S2 antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded human esophagus tissue labeling Cytokeratin 17 (HA720138F) and Vimentin (EM0401).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 17 (HA720138F, green) at 1/200 dilution and Vimentin (EM0401, red) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.
iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain. -
Immunocytochemistry analysis of Hela cells labeling Cytokeratin 17 with Rabbit anti-Cytokeratin 17 antibody (HA720138F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 1% BSA for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Cytokeratin 17 antibody (HA720138F) at 1/100 dilution in 1% BSA overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"