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iFluor™ 647 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]

Usd: 429

Specification

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Catalog# HA720146F

iFluor™ 647 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]

Application

  • IF-Tissue

Reactivity

  • Human

  • Rat

  • Mouse

Conjugation

  • iFluor™ 647

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

iFluor™ 647 Conjugated Cytokeratin 10 Recombinant Rabbit Monoclonal Antibody [ST05-43]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Cytokeratin 10 aa 541-584 / 584.

Species Reactivity

Human, Rat, Mouse

Validated Applications

IF-Tissue

Molecular Weight

Predicted band size: 59 kDa

Positive Control

Human skin tissue, rat skin tissue.

Conjugation

iFluor™ 647

Clone Number

ST05-43

RRID

AB_3072084

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Tissue

  • 1:50

Target

Function

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors. Cytokeratins 10 and 13 are present in the cytoskeletal region of a subset of squamous cell carcinomas. Cytokeratin 10 is a heterotetramer of two type I and two type II keratins, is generally associated with keratin 1, and is seen in all suprabasal cell layers including stratum corneum.

Background References

1. Tabuchi Y. et al. Development of oral epithelial cell line ROE2 with differentiation potential from transgenic rats harboring temperature-sensitive simian virus40 large T-antigen gene. Exp Anim 63:31-44 (2014).

2. Zhu XJ. et al. BMP-FGF signaling axis mediates Wnt-induced epidermal stratification in developing mammalian skin. PLoS Genet 10:e1004687 (2014).

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P13645 Human

SwissProt: Q6IFW6 Rat

Synonyms

BCIE antibody

BIE antibody

CK 10 antibody

CK10 antibody

CK-10 antibody

Cytokeratin-10 antibody

EHK antibody

K10 antibody

K1C10_HUMAN antibody

Keratin 10 antibody

Expand

Images

  • Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (<a href="/products/HA720146F" style="font-weight: bold;text-decoration: underline;">HA720146F</a>), Cytokeratin 15 (<a href="/products/HA720136F" style="font-weight: bold;text-decoration: underline;">HA720136F</a>) and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (<a href="/products/HA720146F" style="font-weight: bold;text-decoration: underline;">HA720146F</a>, red) at 1/50 dilution, Cytokeratin 15 (<a href="/products/HA720136F" style="font-weight: bold;text-decoration: underline;">HA720136F</a>, green) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />Alexa Fluor&reg; 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded human skin tissue labeling Cytokeratin 10 (HA720146F), Cytokeratin 15 (HA720136F) and Vimentin (EM0401).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720146F, red) at 1/50 dilution, Cytokeratin 15 (HA720136F, green) at 1/200 dilution and Vimentin (EM0401, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

  • Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (<a href="/products/HA720146F" style="font-weight: bold;text-decoration: underline;">HA720146F</a>), Cytokeratin 15 (<a href="/products/HA720136F" style="font-weight: bold;text-decoration: underline;">HA720136F</a>) and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>).<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (<a href="/products/HA720146F" style="font-weight: bold;text-decoration: underline;">HA720146F</a>, red) at 1/50 dilution, Cytokeratin 15 (<a href="/products/HA720136F" style="font-weight: bold;text-decoration: underline;">HA720136F</a>, green) at 1/200 dilution and Vimentin (<a href="/products/EM0401" style="font-weight: bold;text-decoration: underline;">EM0401</a>, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.<br /><br />Alexa Fluor&reg; 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

    Immunofluorescence analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 10 (HA720146F), Cytokeratin 15 (HA720136F) and Vimentin (EM0401).

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Cytokeratin 10 (HA720146F, red) at 1/50 dilution, Cytokeratin 15 (HA720136F, green) at 1/200 dilution and Vimentin (EM0401, magenta) at 1/1,000 dilution overnight at 4 ℃, washed with PBS.

    Alexa Fluor® 555 conjugate-Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation