iFluor™ 488 Conjugated Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
Catalog# HA720155F
iFluor™ 488 Conjugated Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
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IF-Cell
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IF-Tissue
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Human
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Mouse
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Rat
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Collagen VI aa 17-255 / 1,028.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Cell, IF-Tissue
Molecular Weight
Predicted band size: 109 kDa
Positive Control
Hela, mouse colon tissue.
Conjugation
iFluor™ 488
Clone Number
SD83-03
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:100
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IF-Tissue
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1:50
Target
Function
This antibody is well suited to detect extracellular matrix proteins in normal as well as disease state tissues. Disruption of tissue organization is the hallmark of neoplasia. Malignant lesions can be distinguished from benign by examining the breakdown of basement membranes and loss of 3-dimensional architecture. Malignant cells are presumed to use matrix metalloproteases to degrade barriers created by the extracellular matrix which then allows metastasis to occur. Collagenases, stomelysins and gelatinases can collectively degrade all of the various components of the extracellular matrix, including fibrillar and non-fibrillar collagens and basement membrane glycoproteins.
Background References
1. Kristofik N et al. Impaired von Willebrand factor adhesion and platelet response in thrombospondin-2 knockout mice. Blood 128:1642-50 (2016).
2. Okawa S et al. Lipopolysaccharide induces expression of collagen VI in the rat lung. J Toxicol Pathol 28:37-41 (2015).
Subcellular Location
Extracellular matrix.
UNIPROT
Synonyms
Alpha 1 (VI) chain (61 AA) antibody
CO6A1_HUMAN antibody
COL6A1 antibody
COL6A2 antibody
COL6A3 antibody
Collagen alpha 2(VI) chain antibody
Collagen alpha 3(VI) chain antibody
Collagen alpha-1(VI) chain antibody
Collagen type VI alpha 1 antibody
Collagen type VI alpha 2 antibody
ExpandImages
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Immunocytochemistry analysis of Hela cells labeling Collagen VI with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-Collagen VI antibody (HA720155F) at 1/100 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. -
Immunofluorescence analysis of paraffin-embedded mouse colon tissue labeling Collagen VI (HA720155F).
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody Collagen VI (HA720155F, iFluor™ 488) at 1/50 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Collagen VI Recombinant Rabbit Monoclonal Antibody [SD83-03] - BSA and Azide free
Application: WB,IF-Cell,IF-Tissue,IHC-P
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
