iFluor™ 488 Conjugated FOXO1A Recombinant Rabbit Monoclonal Antibody [SU33-01]
Catalog# HA720161F
iFluor™ 488 Conjugated FOXO1A Recombinant Rabbit Monoclonal Antibody [SU33-01]
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IF-Cell
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FC
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Human
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Mouse
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Rat
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iFluor™ 488
Overview
Product Name
iFluor™ 488 Conjugated FOXO1A Recombinant Rabbit Monoclonal Antibody [SU33-01]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human FOXO1A aa 301-350 / 655.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Cell, FC
Molecular Weight
Predicted band size: 70 kDa
Positive Control
Hela, NIH/3T3.
Conjugation
iFluor™ 488
Clone Number
SU33-01
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:50
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FC
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1:500-1:1,000
Target
Function
FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is a ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas. These translocations result in the fusion of the amino terminus of Pax-3 or Pax-7, including the paired box and homeodomain DNA-binding domains, with the carboxy-terminus of FKHR, which contains a transcriptional activation domain. The Pax-3/FKHR fusion protein appears to function as an oncogenic transcription factor that enhances the activation of normal Pax-3 target genes.
Background References
1. Xiao N et al. The E3 ubiquitin ligase Itch is required for the differentiation of follicular helper T cells. Nat Immunol 15:657-66 (2014).
2. Chen C et al. High cytoplasmic FOXO1 and pFOXO1 expression in astrocytomas are associated with worse surgical outcome. PLoS One 8:e69260 (2013).
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
FKH 1 antibody
FKH1 antibody
FKHR antibody
Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma) antibody
Forkhead box O1 antibody
Forkhead box protein O1 antibody
Forkhead box protein O1A antibody
Forkhead in rhabdomyosarcoma antibody
Forkhead, Drosophila, homolog of, in rhabdomyosarcoma antibody
FoxO transcription factor antibody
ExpandImages
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Immunocytochemistry analysis of Hela cells labeling FOXO1A with Rabbit anti-FOXO1A antibody (HA720161F) at 1/50 dilution.
Cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% normal goat serum for 1 hour at 37 ℃. Cells were then incubated with Rabbit anti-FOXO1A antibody (HA720161F) at 1/50 dilution in 2% normal goat serum overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) were used as the secondary antibody at 1/800 dilution. -
Flow cytometric analysis of NIH/3T3 cells labeling FOXO1A.
Cells were fixed and permeabilized. Then incubated for 30 minutes at +4℃ with FOXO1A (HA720161F, red, 1ug/ml) and Rabbit IgG Isotype Control (iFluor™ 488, green, 1ug/ml). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
FOXO1A Recombinant Rabbit Monoclonal Antibody [SU33-01]
Application: WB,IF-Cell,IHC-P,FC
Reactivity: Human,Mouse,Rat,Zebrafish
Conjugate: unconjugated
