iFluor™ 647 Conjugated SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44]
Catalog# HA720173F
iFluor™ 647 Conjugated SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44]
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IF-Tissue
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FC
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Human
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Mouse
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Rat
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iFluor™ 647
Overview
Product Name
iFluor™ 647 Conjugated SATB2 Recombinant Rabbit Monoclonal Antibody [JM52-44]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SATB2 238-287 / 733.
Species Reactivity
Human, Mouse, Rat
Validated Applications
IF-Tissue, FC
Molecular Weight
Predicted band size: 83 kDa
Positive Control
Human colon carcinoma tissue, THP-1.
Conjugation
iFluor™ 647
Clone Number
JM52-44
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
Preservative: 0.02% Sodium azide Constituents: 30% Glycerol, 1% BSA, 68.98% PBS
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Tissue
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1:200
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FC
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1:500-1:1,000
Target
Function
SATB2 (Special AT-rich sequence-binding protein 2) is a nuclear matrix protein that influences craniofacial formation mechanisms, such as jaw and palate development, and is part of a transcriptional network regulating skeletal development and osteoblast differentiation. Highly expressed in adult and fetal brain, SATB2 contains two CUT DNA-binding domains and one homeobox domain and is closely related to SATB1, a transcriptional repressor. SATB2 is thought to bind to matrix-attachment regions (MARs) and regulate MAR-dependent transcription of various genes, including HoxA2 and ATF4 (CREB-2), involved in skeletal development. Functioning as both a transcriptional activator and repressor, SATB2 can also act as a protein scaffold that can enhance the activity of other DNA-binding proteins. Defects in the gene encoding SATB2 are the cause of cleft palate manifested in conjunction with severe mental retardation.
Background References
1. Gu W et al. SATB2 preserves colon stem cell identity and mediates ileum-colon conversion via enhancer remodeling. Cell Stem Cell. 2022 Jan
2. Roy SK et al. SATB2 is a novel biomarker and therapeutic target for cancer. J Cell Mol Med. 2020 Oct
Sequence Similarity
Belongs to the CUT homeobox family.
Tissue Specificity
High expression in adult brain, moderate expression in fetal brain, and weak expression in adult liver, kidney, and spinal cord and in select brain regions, including amygdala, corpus callosum, caudate nucleus, and hippocampus.
Post-translational Modification
Sumoylated by PIAS1. Sumoylation promotes nuclear localization, but represses transcription factor activity.
Subcellular Location
Nucleus matrix.
UNIPROT
Synonyms
DNA binding protein SATB2 antibody
DNA-binding protein SATB2 antibody
FLJ21474 antibody
FLJ32076 antibody
GLSS antibody
KIAA1034 antibody
MGC119474 antibody
MGC119477 antibody
SATB family member 2 antibody
SATB homeobox 2 antibody
ExpandImages
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Immunofluorescence analysis of paraffin-embedded human colon carcinoma tissue labeling SATB2 (HA720173F).
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibody SATB2 (HA720173F, iFluor™ 647) at 1/200 dilution overnight at 4 ℃, washed with PBS. DAPI was used as nuclear counterstain. -
Flow cytometric analysis of THP-1 cells labeling SATB2.
Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with SATB2 (HA720173F, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 647, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"