PE Conjugated Mouse CD16/32 Recombinant Rabbit Monoclonal Antibody [PSH06-60]
Catalog# HA720216F
PE Conjugated Mouse CD16/32 Recombinant Rabbit Monoclonal Antibody [PSH06-60]
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FC
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Mouse
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PE
Overview
Product Name
PE Conjugated Mouse CD16/32 Recombinant Rabbit Monoclonal Antibody [PSH06-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within
Species Reactivity
Mouse
Validated Applications
FC
Molecular Weight
Predicted band size: 30/37 kDa
Positive Control
Mouse spleen cells.
Conjugation
PE
Clone Number
PSH06-60
Product Features
Form
Liquid
Concentration
(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2℃ to 8℃. Avoid repeated freeze / thaw cycles.
Storage Buffer
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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FC
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5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Target
Function
All of the Fcγ receptors (FcγR) belong to the immunoglobulin superfamily and are the most important Fc receptors for inducing phagocytosis of opsonized (marked) microbes. This family includes several members, FcγRI (CD64), FcγRIIA (CD32), FcγRIIB (CD32), FcγRIIIA (CD16a), FcγRIIIB (CD16b), which differ in their antibody affinities due to their different molecular structure. For instance, FcγRI binds to IgG more strongly than FcγRII or FcγRIII does. FcγRI also has an extracellular portion composed of three immunoglobulin (Ig)-like domains, one more domain than FcγRII or FcγRIII has. This property allows FcγRI to bind a sole IgG molecule (or monomer), but all Fcγ receptors must bind multiple IgG molecules within an immune complex to be activated. The Fc-gamma receptors differ in their affinity for IgG and likewise the different IgG subclasses have unique affinities for each of the Fc gamma receptors. These interactions are further tuned by the glycan (oligosaccharide) at position CH2-84.4 of IgG. For example, by creating steric hindrance, fucose containing CH2-84.4 glycans reduce IgG affinity for FcγRIIIA. In contrast, G0 glycans, which lack galactose and terminate instead with GlcNAc moieties, have increased affinity for FcγRIIIA.
Background References
1. Xu J et al. CD16 and CD32 Gene Polymorphisms May Contribute to Risk of Idiopathic Thrombocytopenic Purpura. Med Sci Monit. 2016 Jun
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
CD16a antibody
CD16b antibody
CD32 antibody
CD32A antibody
CD32B antibody
CDw32 antibody
Fc fragment of IgG low affinity IIa receptor antibody
Fc fragment of IgG low affinity IIb receptor antibody
Fc fragment of IgG low affinity IIIa receptor antibody
Fc fragment of IgG low affinity IIIb receptor antibody
ExpandImages
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Flow cytometric analysis of mouse spleen cells labeling Mouse CD16/32.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA720216F, 1μg/mL) (red). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"