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FITC Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [PSH10-66]

Usd: 429

Specification

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Catalog# HA720223F

FITC Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [PSH10-66]

Application

  • IF-Cell

  • FC

Reactivity

  • Species independent

Conjugation

  • FITC

Overview

Product Name

FITC Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [PSH10-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein.

Species Reactivity

Species independent

Validated Applications

IF-Cell, FC

Conjugation

FITC

Clone Number

PSH10-66

Reactivity Data

IF-Cell FC
Species Independent
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

Target

Function

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

Background References

1. Liu Z et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR and activating NF-kB pathway in mice adipocytes. Sci Rep 6:21382 (2016).

2. Gauson EJ et al. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication. J Virol 89:4980-91 (2015).

Synonyms

HA epitope tag antibody

HA1 antibody

HA2 antibody

hemagglutinin antibody

Hemagglutinin HA1 chain antibody

Hemagglutinin HA2 chain antibody

Images

  • Immunocytochemistry analysis of 293T cells labeling HA tag with Rabbit anti-HA tag antibody (<a href="/products/HA720223F" style="font-weight: bold;text-decoration: underline;">HA720223F</a>) at 1/100 dilution.<br /><br />293T cells, transfected with empty control (top, negative) / HA-tagged Nanos homolog 3 (middle, positive) / HA-tagged CLK4 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (<a href="/products/HA720223F" style="font-weight: bold;text-decoration: underline;">HA720223F</a>, FITC, green) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Immunocytochemistry analysis of 293T cells labeling HA tag with Rabbit anti-HA tag antibody (HA720223F) at 1/100 dilution.

    293T cells, transfected with empty control (top, negative) / HA-tagged Nanos homolog 3 (middle, positive) / HA-tagged CLK4 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (HA720223F, FITC, green) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

  • Flow cytometric analysis of 293T cells (left) / 293T cells transfected with HA-tagged CLK4 (right) labeling HA tag (<a href="/products/HA720223F" style="font-weight: bold;text-decoration: underline;">HA720223F</a>, FITC).<br /><br />Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with HA tag (<a href="/products/HA720223F" style="font-weight: bold;text-decoration: underline;">HA720223F</a>, FITC, red, 1/1,000) and Rabbit IgG Isotype Control (FITC, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of 293T cells (left) / 293T cells transfected with HA-tagged CLK4 (right) labeling HA tag (HA720223F, FITC).

    Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with HA tag (HA720223F, FITC, red, 1/1,000) and Rabbit IgG Isotype Control (FITC, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"