iFluor™ 594 Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [PSH10-66] (HA720224F)
Catalog# HA720224F
Rabbit Monoclonal to HA tag
Tested for
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IF-Cell
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FC
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to iFluor™ 594
Immunocytochemistry analysis of 293T cells labeling HA tag with Rabbit anti-HA tag antibody (HA720224F) at 1/100 dilution.
293T cells, transfected with empty control (top, negative) / HA-tagged CLK4 (middle, positive) / HA-tagged Nanos homolog 3 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (HA720224F, iFluor™ 594, red) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
293T cells, transfected with empty control (top, negative) / HA-tagged CLK4 (middle, positive) / HA-tagged Nanos homolog 3 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (HA720224F, iFluor™ 594, red) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.