iFluor™ 594 Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [PSH10-66]
Catalog# HA720224F
iFluor™ 594 Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [PSH10-66]
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IF-Cell
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FC
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Species independent
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iFluor™ 594
Overview
Product Name
iFluor™ 594 Conjugated HA tag Recombinant Rabbit Monoclonal Antibody [PSH10-66]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within N Terminal fusion protein and C Terminal fusion protein.
Species Reactivity
Species independent
Validated Applications
IF-Cell, FC
Conjugation
iFluor™ 594
Clone Number
PSH10-66
Reactivity Data
| IF-Cell | FC | |
|---|---|---|
| Species Independent | ||
| Human |
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|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Background References
1. Liu Z et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR and activating NF-kB pathway in mice adipocytes. Sci Rep 6:21382 (2016).
2. Gauson EJ et al. Evidence supporting a role for TopBP1 and Brd4 in the initiation but not continuation of human papillomavirus 16 E1/E2-mediated DNA replication. J Virol 89:4980-91 (2015).
Synonyms
HA epitope tag antibody
HA1 antibody
HA2 antibody
hemagglutinin antibody
Hemagglutinin HA1 chain antibody
Hemagglutinin HA2 chain antibody
Images
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Immunocytochemistry analysis of 293T cells labeling HA tag with Rabbit anti-HA tag antibody (HA720224F) at 1/100 dilution.
293T cells, transfected with empty control (top, negative) / HA-tagged CLK4 (middle, positive) / HA-tagged Nanos homolog 3 (bottom, positive) expression vector, respectively, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-HA tag antibody (HA720224F, iFluor™ 594, red) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI. -
Flow cytometric analysis of 293T cells (left) / 293T cells transfected with HA-tagged CLK4 (right) labeling HA tag (HA720224F, iFluor™ 594).
Cells were fixed and permeabilized. Then incubated for 1 hour at +4℃ with HA tag (HA720224F, iFluor™ 594, red, 1/1,000) and Rabbit IgG Isotype Control (iFluor™ 594, green, 1/1,000). Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"