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FITC Conjugated GST Recombinant Rabbit Monoclonal Antibody [SN70-05]

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Specification

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Catalog# HA720225F

FITC Conjugated GST Recombinant Rabbit Monoclonal Antibody [SN70-05]

Application

  • IF-Cell

Reactivity

  • Species independent

Conjugation

  • FITC

Overview

Product Name

FITC Conjugated GST Recombinant Rabbit Monoclonal Antibody [SN70-05]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

GST tag recombinant protein.

Species Reactivity

Species independent

Validated Applications

IF-Cell

Positive Control

HeLa transfected with GST-tagged Histone H3.1 cells.

Conjugation

FITC

Clone Number

SN70-05

Reactivity Data

IF-Cell
Species Independent
Tested Tested

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

Supplied in phosphate-buffered solution, pH 7.2, containing 0.2% ProClean 950 and BSA.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:100

Target

Function

The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.

Background References

1. Pettit MW et al. Construction and physiochemical characterisation of a multi-composite, potential oral vaccine delivery system (VDS). Int J Pharm 468:264-71 (2014).

2. Zhang D et al. The Chromatin-Remodeling Factor PICKLE Integrates Brassinosteroid and Gibberellin Signaling during Skotomorphogenic Growth in Arabidopsis. Plant Cell 26:2472-2485 (2014).

Synonyms

Glutathione S Transferase antibody

Glutathione S-transferase class-mu 26 kDa isozyme antibody

GST antibody

Sj26 antigen antibody

SjGST antibody

Images

  • Immunocytochemistry analysis of HeLa transfected with GST-tagged Histone H3.1 cells labeling GST with Rabbit anti-GST antibody (<a href="/products/HA720225F" style="font-weight: bold;text-decoration: underline;">HA720225F</a>, FITC) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody (<a href="/products/HA720225F" style="font-weight: bold;text-decoration: underline;">HA720225F</a>, FITC) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Histone H3 (<a href="/products/HA601278" style="font-weight: bold;text-decoration: underline;">HA601278</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa transfected with GST-tagged Histone H3.1 cells labeling GST with Rabbit anti-GST antibody (HA720225F, FITC) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GST antibody (HA720225F, FITC) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Histone H3 (HA601278, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"