iFluor™ 594 Conjugated V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01] (HA720226F)
Catalog# HA720226F
Rabbit Monoclonal to V5 tag
Tested for
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IF-Cell
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Conjugated to iFluor™ 594
Immunocytochemistry analysis of HeLa cells labeling V5 tag with Rabbit anti-V5 tag antibody (HA720226F) at 1/1,000 dilution.
HeLa cells, untransfected (top, negative) / transfected with V5-tagged Histone H3.1 (bottom, positive) expression vector, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-V5 tag antibody (HA720226F, iFluor™ 594, red) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Histone H3 (HA601278, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.
HeLa cells, untransfected (top, negative) / transfected with V5-tagged Histone H3.1 (bottom, positive) expression vector, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-V5 tag antibody (HA720226F, iFluor™ 594, red) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.
Histone H3 (HA601278, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.