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iFluor™ 594 Conjugated V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01]

Usd: 429

Specification

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Catalog# HA720226F

iFluor™ 594 Conjugated V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01]

Application

  • IF-Cell

Reactivity

  • Species independent

Conjugation

  • iFluor™ 594

Overview

Product Name

iFluor™ 594 Conjugated V5 tag Recombinant Rabbit Monoclonal Antibody [SY30-01]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide immune sequence is CGKPIPNPLLGLDST.

Species Reactivity

Species independent

Validated Applications

IF-Cell

Conjugation

iFluor™ 594

Clone Number

SY30-01

Reactivity Data

IF-Cell
Species Independent
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 1% BSA, 40% Glycerol, 0.2% Proclean 950.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IF-Cell

  • 1:1,000

Target

Function

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The anti-V5 antibody detects recombinant proteins containing the 14 amino acid GKPIPNPLLGLDST sequence.

Background References

1. Tatenhorst L et al. Fasudil attenuates aggregation of a-synuclein in models of Parkinson\'s disease. Acta Neuropathol Commun 4:39 (2016).

2. Ji H et al. EGFR phosphorylates FAM129B to promote Ras activation. Proc Natl Acad Sci U S A 113:644-9 (2016).

Synonyms

GKPIPNPLLGLDST epitope tag antibody

GKPIPNPLLGLDST tag antibody

Protein Rev antibody

Regulator of expression of viral proteins antibody

rev antibody

V5 epitope tag antibody

Images

  • Immunocytochemistry analysis of HeLa cells labeling V5 tag with Rabbit anti-V5 tag antibody (<a href="/products/HA720226F" style="font-weight: bold;text-decoration: underline;">HA720226F</a>) at 1/1,000 dilution.<br /><br />HeLa cells, untransfected (top, negative) / transfected with V5-tagged Histone H3.1 (bottom, positive) expression vector, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-V5 tag antibody (<a href="/products/HA720226F" style="font-weight: bold;text-decoration: underline;">HA720226F</a>, iFluor&trade; 594, red) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.<br /><br />Histone H3 (<a href="/products/HA601278" style="font-weight: bold;text-decoration: underline;">HA601278</a>, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 488, <a href="/products/HA1125" style="font-weight: bold;text-decoration: underline;">HA1125</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling V5 tag with Rabbit anti-V5 tag antibody (HA720226F) at 1/1,000 dilution.

    HeLa cells, untransfected (top, negative) / transfected with V5-tagged Histone H3.1 (bottom, positive) expression vector, were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-V5 tag antibody (HA720226F, iFluor™ 594, red) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Nuclear DNA was labelled in blue with DAPI.

    Histone H3 (HA601278, green) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"