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GNA13 Recombinant Rabbit Monoclonal Antibody [JE63-76]

Usd: 385

Specification

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Catalog# HA721007

GNA13 Recombinant Rabbit Monoclonal Antibody [JE63-76]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

GNA13 Recombinant Rabbit Monoclonal Antibody [JE63-76]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human GNA13 aa 278-377/377.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 44 kDa

Positive Control

293T cell lysate, HepG2 cell lysate, 293 cell lysate, Jurkat cell lysate, PC-12 cell lysate, NIH/3T3 cell lysates, mouse colon tissue.

Conjugation

unconjugated

Clone Number

JE63-76

RRID

AB_3072132

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:500

Target

Function

Guanine nucleotide-binding protein subunit alpha-13 is a protein that in humans is encoded by the GNA13 gene. The GNA13 gene encodes the G13 G protein alpha subunit. Together with GNA12, these two proteins comprise one of the four classes of heterotrimeric G protein alpha subunits. Heterotrimeric G proteins function in transducing hormone and neurotransmitter signals detected by cell surface G protein-coupled receptors to intracellular signaling pathways to modulate cell functions. G protein alpha subunits bind to guanine nucleotides and function in a regulatory cycle, and are active when bound to GTP but inactive and associated with the G beta-gamma complex when bound to GDP. Active GTP-bound G12 alpha subunit interacts with and activates ARHGEF1, ARHGEF11, and ARHGEF12. These ARHGEF proteins function as guanine nucleotide exchange factors for the Rho small GTPases to regulate the actin cytoskeleton. GNA13 has been shown to interact with AKAP3, RIC8A, and Radixin. Recurrent mutations in this gene have been associated to cases of diffuse large B-cell lymphoma.

Background References

1. Xia Z. et. al. GNA13 regulates BCL2 expression and the sensitivity of GCB-DLBCL cells to BCL2 inhibitors in a palmitoylation-dependent manner. Cell Death Dis. 2021 Jan

2. Rasheed SAK. et. al. GNA13 expression promotes drug resistance and tumor-initiating phenotypes in squamous cell cancers. Oncogene. 2018 Mar

Subcellular Location

Cell membrane, Nucleus, Cytoplasm, Melanosome.

UNIPROT

SwissProt: Q14344 Human

SwissProt: P27601 Mouse

SwissProt: Q6Q7Y5 Rat

Synonyms

G alpha 13 antibody

G alpha-13 antibody

G-protein subunit alpha-13 antibody

GNA13 antibody

GNA13_HUMAN antibody

Guanine nucleotide binding protein (G protein) alpha 13 antibody

Guanine nucleotide binding protein alpha 13 subunit antibody

Guanine nucleotide-binding protein subunit alpha-13 antibody

Images

  • Western blot analysis of GNA13 on different lysates with Rabbit anti-GNA13 antibody (<a href="/products/HA721007" style="font-weight: bold;text-decoration: underline;">HA721007</a>) at 1/500 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: 293 cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: PC-12 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721007" style="font-weight: bold;text-decoration: underline;">HA721007</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GNA13 on different lysates with Rabbit anti-GNA13 antibody (HA721007) at 1/500 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: 293 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: PC-12 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 40 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721007) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of GNA13 on NIH/3T3 cell lysates with Rabbit anti-GNA13 antibody (<a href="/products/HA721007" style="font-weight: bold;text-decoration: underline;">HA721007</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 44 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721007" style="font-weight: bold;text-decoration: underline;">HA721007</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of GNA13 on NIH/3T3 cell lysates with Rabbit anti-GNA13 antibody (HA721007) at 1/1,000 dilution.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 44 kDa
    Observed band size: 40 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721007) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-GNA13 antibody (<a href="/products/HA721007" style="font-weight: bold;text-decoration: underline;">HA721007</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721007" style="font-weight: bold;text-decoration: underline;">HA721007</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-GNA13 antibody (HA721007) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721007) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation