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SLP-2 Recombinant Rabbit Monoclonal Antibody [JE63-15]

Usd: 385

Specification

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Catalog# HA721009

SLP-2 Recombinant Rabbit Monoclonal Antibody [JE63-15]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SLP-2 Recombinant Rabbit Monoclonal Antibody [JE63-15]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SLP-2 aa 1-200/356.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 39 kDa

Positive Control

HAP1-parental cell lysate, HAP1-商品名 KD cell lysate, HeLa, human kidney tissue, rat testis tissue, Hela cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

JE63-15

RRID

AB_3072134

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:400-1:1,000

  • IF-Cell

  • 1:50

Target

Function

Mitochondrial protein that probably regulates the biogenesis and the activity of mitochondria. Stimulates cardiolipin biosynthesis, binds cardiolipin-enriched membranes where it recruits and stabilizes some proteins including prohibitin and may therefore act in the organization of functional microdomains in mitochondrial membranes. Through regulation of the mitochondrial function may play a role into several biological processes including cell migration, cell proliferation, T-cell activation, calcium homeostasis and cellular response to stress. May play a role in calcium homeostasis through negative regulation of calcium efflux from mitochondria. Required for mitochondrial hyperfusion a pro-survival cellular response to stress which results in increased ATP production by mitochondria. May also regulate the organization of functional domains at the plasma membrane and play a role in T-cell activation through association with the T-cell receptor signaling complex and its regulation.

Background References

1. Guo XY. et. al. Clinical significance of SLP-2 in epithelial ovarian cancer and its regulatory effect on the Notch signaling pathway. Eur Rev Med Pharmacol Sci. 2020 Feb

2. Zhang J. et. al. Expression and clinical significance of SLP-2 in ovarian tumors. Oncol Lett. 2019 May

Subcellular Location

Mitochondrion, Mitochondrion inner membrane, Mitochondrion intermembrane space, Cytoskeleton, Cell membrane, Membrane raft.

UNIPROT

SwissProt: Q9UJZ1 Human

SwissProt: Q99JB2 Mouse

SwissProt: Q4FZT0 Rat

Synonyms

EPB72 like protein 2 antibody

EPB72-like protein 2 antibody

HSPC108 antibody

OTTHUMP00000021320 antibody

Paraprotein target 7 antibody

Paratarg 7 antibody

SLP 2 antibody

SLP-2 antibody

SLP2 antibody

STML2_HUMAN antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of SLP-2 on different lysates with Rabbit anti-SLP-2 antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-SLP-2 KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 39 kDa<br />Observed band size: 39 kDa<br /><br />Exposure time: 9 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of SLP-2 on different lysates with Rabbit anti-SLP-2 antibody (HA721009) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-SLP-2 KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

    Exposure time: 9 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721009) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling SLP-2 with Rabbit anti-SLP-2 antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLP-2 antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling SLP-2 with Rabbit anti-SLP-2 antibody (HA721009) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-SLP-2 antibody (HA721009) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SLP-2 antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-SLP-2 antibody (HA721009) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721009) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SLP-2 antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat testis tissue with Rabbit anti-SLP-2 antibody (HA721009) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721009) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of SLP-2 on different lysates with Rabbit anti-SLP-2 antibody (<a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>) at 1/5,000 dilution.<br /><br />Lane 1: Hela cell lysate<br />Lane 2: Jurkat cell lysate<br />Lane 3: NIH/3T3 cell lysate<br />Lane 4: PC-12 cell lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 9 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721009" style="font-weight: bold;text-decoration: underline;">HA721009</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 39 kDa<br />Observed band size: 39 kDa

    Western blot analysis of SLP-2 on different lysates with Rabbit anti-SLP-2 antibody (HA721009) at 1/5,000 dilution.

    Lane 1: Hela cell lysate
    Lane 2: Jurkat cell lysate
    Lane 3: NIH/3T3 cell lysate
    Lane 4: PC-12 cell lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 9 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721009, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 39 kDa
    Observed band size: 39 kDa

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