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CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21]

Usd: 385

Specification

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Catalog# HA721013

CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

CD100 Recombinant Rabbit Monoclonal Antibody [JE63-21]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CD100 aa 126-175/862.

Species Reactivity

Human

Validated Applications

WB

Molecular Weight

Predicted band size: 96 kDa

Positive Control

Jurkat cell lysate, Daudi cell lysate, HL-60 cell lysate.

Conjugation

unconjugated

Clone Number

JE63-21

RRID

AB_3072137

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

Target

Function

Cell surface receptor for PLXNB1 and PLXNB2 that plays an important role in cell-cell signaling. Regulates GABAergic synapse development (By similarity). Promotes the development of inhibitory synapses in a PLXNB1-dependent manner (By similarity). Modulates the complexity and arborization of developing neurites in hippocampal neurons by activating PLXNB1 and interaction with PLXNB1 mediates activation of RHOA. Promotes the migration of cerebellar granule cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Induces endothelial cell migration through the activation of PTK2B/PYK2, SRC, and the phosphatidylinositol 3-kinase-AKT pathway.

Background References

1. Wang L. et. al. The emerging roles of semaphorin4D/CD100 in immunological diseases. Biochem Soc Trans. 2020 Dec

2. Kalina T. et. al. CD Maps-Dynamic Profiling of CD1-CD100 Surface Expression on Human Leukocyte and Lymphocyte Subsets. Front Immunol. 2019 Oct

Subcellular Location

Cell membrane.

UNIPROT

SwissProt: Q92854 Human

Synonyms

A8 antibody

BB 18 antibody

BB18 antibody

CD 100 antibody

CD100 antibody

CD100 antigen antibody

Coll 4 antibody

COLL4 antibody

Collapsin 4 antibody

Collapsin4 antibody

Expand

Images

  • Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (<a href="/products/HA721013" style="font-weight: bold;text-decoration: underline;">HA721013</a>) at 1/500 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: Daudi cell lysate<br />Lane 3: HL-60 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 96 kDa<br />Observed band size: 130 kDa<br /><br />Exposure time: 30 seconds;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721013" style="font-weight: bold;text-decoration: underline;">HA721013</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (HA721013) at 1/500 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: Daudi cell lysate
    Lane 3: HL-60 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 96 kDa
    Observed band size: 130 kDa

    Exposure time: 30 seconds;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721013) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (<a href="/products/HA721013" style="font-weight: bold;text-decoration: underline;">HA721013</a>) at 1/2,000 dilution. <br /><br />Lane 1: HAP1-si NT cell lysate <br />Lane 2: HAP1-si CD100 cell lysate <br /><br />Lysates/proteins at 10 µg/Lane. <br /><br />Predicted band size: 96 kDa <br />Observed band size: 130 kDa <br /><br />Exposure time: 62 seconds; ECL: K1802; <br /><br />4-20% SDS-PAGE gel. <br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721013" style="font-weight: bold;text-decoration: underline;">HA721013</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of CD100 on different lysates with Rabbit anti-CD100 antibody (HA721013) at 1/2,000 dilution.

    Lane 1: HAP1-si NT cell lysate
    Lane 2: HAP1-si CD100 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 96 kDa
    Observed band size: 130 kDa

    Exposure time: 62 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721013) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

CD100 Mouse Monoclonal Antibody [5H6E3]

Application: WB,IHC-P,FC

Reactivity: Human

Conjugate: unconjugated