SLC45A3 Recombinant Rabbit Monoclonal Antibody [JE58-86]
Catalog# HA721018
SLC45A3 Recombinant Rabbit Monoclonal Antibody [JE58-86]
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
SLC45A3 Recombinant Rabbit Monoclonal Antibody [JE58-86]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human SLC45A3 aa 400-500.
Species Reactivity
Human
Validated Applications
IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 59 kDa
Positive Control
Human prostate tissue, human prostate cancer tissue.
Conjugation
unconjugated
Clone Number
JE58-86
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:200
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IF-Tissue
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1:200
Target
Function
Solute carrier family 45 member 3 (SLC45A3), also known as prostate cancer-associated protein 6 or prostein, is a protein that in humans is encoded by the SLC45A3 gene. SLC45A3 is an androgen-regulated gene and a prostate specific marker expressed in prostatic glandular cells. SLC45A3 is the second most common gene fusion partner in prostate cancers. SLC45A3-ERG and SLC45A3-ELK4 fusion transcripts have been detected in prostate cancer. Loss of prostein expression in SLC45A3-ERG tumors are significantly associated with high Gleason scores and poor clinical outcomes. Prostein immunoreactivity is seen in the majority of primary and metastatic prostate carcinomas. Staining for prostein in conjunction with PSA improves identification of prostatic origin in unknown primary lesions, when compared against prostein or PSA staining alone.
Background References
1. Qin F. et. al. SLC45A3-ELK4 functions as a long non-coding chimeric RNA. Cancer Lett. 2017 Sep
2. Hernández-Llodrà S. et. al. ERG overexpression plus SLC45A3 (prostein) and PTEN expression loss: Strong association of the triple hit phenotype with an aggressive pathway of prostate cancer progression. Oncotarget. 2017 May
Subcellular Location
Membrane.
UNIPROT
Synonyms
IPCA 2 antibody
IPCA 8 antibody
IPCA6 antibody
PCANAP2 antibody
PCANAP6 antibody
PCANAP8 antibody
Prostate cancer associated gene 2 antibody
Prostate cancer associated gene 6 antibody
Prostate cancer associated gene 8 antibody
Prostate cancer associated protein 2 antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-SLC45A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721018, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-SLC45A3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721018, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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Application: IF-Tissue
Species: Human
Site: prostate cancer
Sample: Paraffin-embedded section
Antibody concentration: 1/200
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"