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DENN Recombinant Rabbit Monoclonal Antibody [JE63-62]

Usd: 385

Specification

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Catalog# HA721022

DENN Recombinant Rabbit Monoclonal Antibody [JE63-62]

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

DENN Recombinant Rabbit Monoclonal Antibody [JE63-62]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human DENN aa 1,598-1,647/1,647.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 183 kDa

Positive Control

HeLa cell lysate, Mouse brain tissue lysate.

Conjugation

unconjugated

Clone Number

JE63-62

RRID

AB_3072146

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

MAP kinase-activating death domain protein is an enzyme that in humans is encoded by the MADD gene. Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene.

Background References

1. Mereis M. et. al. Disorders of flavin adenine dinucleotide metabolism: MADD and related deficiencies. Int J Biochem Cell Biol. 2021 Mar

2. Saini S. et. al. MADD silencing enhances anti-tumor activity of TRAIL in anaplastic thyroid cancer. Endocr Relat Cancer. 2019 Jun

Subcellular Location

Cell membrane, cytoplasm, axon.

UNIPROT

SwissProt: Q8WXG6 Human

Synonyms

Differentially expressed in normal and neoplastic cells antibody

FLJ35600 antibody

IG20 antibody

Insulinoma glucagonoma clone 20 antibody

KIAA0358 antibody

MADD antibody

MADD_HUMAN antibody

MAP kinase-activating death domain protein antibody

Rab3 GDP/GTP exchange factor antibody

RAB3GEP antibody

Expand

Images

  • Western blot analysis of DENN on different lysates with Rabbit anti-DENN antibody (<a href="/products/HA721022" style="font-weight: bold;text-decoration: underline;">HA721022</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate (negative) (20 µg/Lane)<br />Lane 2: Mouse brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 183 kDa<br />Observed band size: 250 kDa<br /><br />Exposure time: 46 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721022" style="font-weight: bold;text-decoration: underline;">HA721022</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of DENN on different lysates with Rabbit anti-DENN antibody (HA721022) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate (negative) (20 µg/Lane)
    Lane 2: Mouse brain tissue lysate (40 µg/Lane)

    Predicted band size: 183 kDa
    Observed band size: 250 kDa

    Exposure time: 46 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"