DENN Recombinant Rabbit Monoclonal Antibody [JE63-62]
Catalog# HA721022
DENN Recombinant Rabbit Monoclonal Antibody [JE63-62]
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WB
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Human
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Mouse
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unconjugated
Overview
Product Name
DENN Recombinant Rabbit Monoclonal Antibody [JE63-62]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human DENN aa 1,598-1,647/1,647.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 183 kDa
Positive Control
HeLa cell lysate, Mouse brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE63-62
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
MAP kinase-activating death domain protein is an enzyme that in humans is encoded by the MADD gene. Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene.
Background References
1. Mereis M. et. al. Disorders of flavin adenine dinucleotide metabolism: MADD and related deficiencies. Int J Biochem Cell Biol. 2021 Mar
2. Saini S. et. al. MADD silencing enhances anti-tumor activity of TRAIL in anaplastic thyroid cancer. Endocr Relat Cancer. 2019 Jun
Subcellular Location
Cell membrane, cytoplasm, axon.
UNIPROT
Synonyms
Differentially expressed in normal and neoplastic cells antibody
FLJ35600 antibody
IG20 antibody
Insulinoma glucagonoma clone 20 antibody
KIAA0358 antibody
MADD antibody
MADD_HUMAN antibody
MAP kinase-activating death domain protein antibody
Rab3 GDP/GTP exchange factor antibody
RAB3GEP antibody
ExpandImages
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Western blot analysis of DENN on different lysates with Rabbit anti-DENN antibody (HA721022) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (negative) (20 µg/Lane)
Lane 2: Mouse brain tissue lysate (40 µg/Lane)
Predicted band size: 183 kDa
Observed band size: 250 kDa
Exposure time: 46 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721022) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"