SARA Recombinant Rabbit Monoclonal Antibody [JE63-65]
Catalog# HA721023
SARA Recombinant Rabbit Monoclonal Antibody [JE63-65]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
SARA Recombinant Rabbit Monoclonal Antibody [JE63-65]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SARA aa 1-50/1,425.
Species Reactivity
Human, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 156 kDa
Positive Control
HUVEC cell lysate, HeLa cell lysate, SiHa cell lysate, Jurkat cell lysate, MCF7 cell lysate, Rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE63-65
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization. This gene encodes a double zinc finger motif-containing protein that participates in the transforming growth factor-beta (TGFB) signalling pathway. The encoded protein interacts directly with SMAD2 and SMAD3, and recruits SMAD2 to the TGFB receptor. There are multiple pseudogenes for this gene on chromosomes 2, 15, and X. Alternative splicing results in multiple transcript variants.
Background References
1. Wojtowicz S. et. al. SMURF2 and SMAD7 induce SARA degradation via the proteasome. Cell Signal. 2020 Aug
2. Rozés-Salvador V. et. al. Fine-Tuning the TGFbeta Signaling Pathway by SARA During Neuronal Development. Front Cell Dev Biol. 2020 Sep
Subcellular Location
Early endosome membrane, Cytoplasm.
Synonyms
CG15667 antibody
hSARA antibody
LD33044p antibody
Madh-interacting protein antibody
MADHIP antibody
Mothers against decapentaplegic homolog-interacting protein antibody
Novel serine protease antibody
NSP antibody
Receptor activation anchor antibody
SAR1 antibody
ExpandImages
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Western blot analysis of SARA on different lysates with Rabbit anti-SARA antibody (HA721023) at 1/1,000 dilution.
Lane 1: HUVEC cell lysate
Lane 2: HeLa cell lysate
Lane 3: SiHa cell lysate
Lane 4: Jurkat cell lysate
Lane 5: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 156 kDa
Observed band size: 156 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721023) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"