SPINK5 / LEKTI Recombinant Rabbit Monoclonal Antibody [JE63-90]
Catalog# HA721027
SPINK5 / LEKTI Recombinant Rabbit Monoclonal Antibody [JE63-90]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
SPINK5 / LEKTI Recombinant Rabbit Monoclonal Antibody [JE63-90]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human LEKTI aa 151-200/1,064.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 121 kDa
Positive Control
HeLa cell lysate, Mouse heart tissue lysate, Rat heart tissue lysate.
Conjugation
unconjugated
Clone Number
JE63-90
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
LEKTI is a large multidomain serine protease inhibitor expressed in stratified epithelial tissue. It consists of 15 domains that are cleaved into smaller, functional fragments by the protease furin. Only two of these domains (2 and 15) contain 6 evenly spaced cysteines responsible for 3 intramolecular disulfide bonds characteristic of Kazal-type related inhibitors. The remaining domains contain 4 cysteines. These disulfide bonds force the molecule into a rigid conformation that enables the protein to interact with a target protease via an extended beta-sheet. All domains (excepting 1, 2 and 15) contain an arginine at P1, indicating trypsin-like proteases are the likely targets. In the epidermis, LEKTI is implicated in the regulation of desquamation via its ability to selectively inhibit KLK5, KLK7 and KLK14. Recombinant full length LEKTI inhibits the exogenous serine proteases trypsin, plasmin, subtilisin A, cathepsin G and human neutrophil elastase. Mutations in the SPINK5 gene result in Netherton syndrome, a disorder characterized by ichthyosis and specific immune system defects.
Background References
1. Park NJ. et. al. Compound K improves skin barrier function by increasing SPINK5 expression. J Ginseng Res. 2020 Nov
2. Lv Z. et. al. A Novel Tumor Suppressor SPINK5 Serves as an Independent Prognostic Predictor for Patients with Head and Neck Squamous Cell Carcinoma. Cancer Manag Res. 2020 Jun
Subcellular Location
Secreted.
Synonyms
Hemofiltrate peptide HF7665 antibody
ISK5_HUMAN antibody
LEKTI antibody
Lympho-epithelial Kazal-type-related inhibitor antibody
Lymphoepithelial Kazal type related inhibitor antibody
NETS antibody
NS antibody
Serine peptidase inhibitor Kazal type 5 antibody
SPINK5 antibody
VAKTI antibody
Images
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Western blot analysis of SPINK5 / LEKTI on different lysates with Rabbit anti-SPINK5 / LEKTI antibody (HA721027) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (40 µg/Lane)
Lane 3: Rat heart tissue lysate (40 µg/Lane)
Predicted band size: 121 kDa
Observed band size: 121 kDa
Exposure time: 51 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721027) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"