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FDPS Recombinant Rabbit Monoclonal Antibody [JE63-33]

Usd: 385

Specification

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Catalog# HA721031

FDPS Recombinant Rabbit Monoclonal Antibody [JE63-33]

Application

  • WB

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

FDPS Recombinant Rabbit Monoclonal Antibody [JE63-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human FDPS aa 370-419/419.

Species Reactivity

Human

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 48 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, THP-1.

Conjugation

unconjugated

Clone Number

JE63-33

RRID

AB_3072155

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • FC

  • 1:500-1:1,000

Target

Function

This gene encodes an enzyme that catalyzes the production of geranyl pyrophosphate and farnesyl pyrophosphate from isopentenyl pyrophosphate and dimethylallyl pyrophosphate. The resulting product, farnesyl pyrophosphate, is a key intermediate in cholesterol and sterol biosynthesis, a substrate for protein farnesylation and geranylgeranylation, and a ligand or agonist for certain hormone receptors and growth receptors. Drugs that inhibit this enzyme prevent the post-translational modifications of small GTPases and have been used to treat diseases related to bone resorption. Multiple pseudogenes have been found on chromosomes 1, 7, 14, 15, 21 and X. Multiple transcript variants encoding different isoforms have been found for this gene.

Background References

1. Chen Z. et. al. FDPS promotes glioma growth and macrophage recruitment by regulating CCL20 via Wnt/beta-catenin signalling pathway. J Cell Mol Med. 2020 Aug

2. Seshacharyulu P. et. al. FDPS cooperates with PTEN loss to promote prostate cancer progression through modulation of small GTPases/AKT axis. Oncogene. 2019 Jun

Subcellular Location

Cytoplasm.

UNIPROT

SwissProt: P14324 Human

Synonyms

(2E antibody

(2E,6E) farnesyl diphosphate synthase antibody

6E)-farnesyl diphosphate synthase antibody

Dimethylallyltranstransferase antibody

Farnesyl diphosphate synthase antibody

Farnesyl diphosphate synthetase antibody

Farnesyl pyrophosphate synthase antibody

Farnesyl pyrophosphate synthetase antibody

Fdps antibody

FPP synthase antibody

Expand

Images

  • Western blot analysis of FDPS on different lysates with Rabbit anti-FDPS antibody (<a href="/products/HA721031" style="font-weight: bold;text-decoration: underline;">HA721031</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HeLa cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721031" style="font-weight: bold;text-decoration: underline;">HA721031</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FDPS on different lysates with Rabbit anti-FDPS antibody (HA721031) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HeLa cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: 40 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721031) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of FDPS on different lysates with Rabbit anti-FDPS antibody (<a href="/products/HA721031" style="font-weight: bold;text-decoration: underline;">HA721031</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-FDPS KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 48 kDa<br />Observed band size: ~38 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721031" style="font-weight: bold;text-decoration: underline;">HA721031</a>) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of FDPS on different lysates with Rabbit anti-FDPS antibody (HA721031) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-FDPS KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 48 kDa
    Observed band size: ~38 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721031) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of THP-1 cells labeling FDPS.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721031" style="font-weight: bold;text-decoration: underline;">HA721031</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of THP-1 cells labeling FDPS.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721031, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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