IL-23 Recombinant Rabbit Monoclonal Antibody [JE38-99]
Catalog# HA721037
IL-23 Recombinant Rabbit Monoclonal Antibody [JE38-99]
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WB
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IHC-P
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
IL-23 Recombinant Rabbit Monoclonal Antibody [JE38-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IL23 100-189.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 21 kDa
Positive Control
Daudi cell lysates, THP-1 cell lysate, Raji cell lysate, human lymph nodes tissue.
Conjugation
unconjugated
Clone Number
JE38-99
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:400
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IF-Tissue
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1:200
Target
Function
This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Background References
1. Moschen AR, et al. IL-12, IL-23 and IL-17 in IBD: immunobiology and therapeutic targeting. Nat Rev Gastroenterol Hepatol. 2019 Mar;16(3):185-196.
2. Hawkes JE, et al. Discovery of the IL-23/IL-17 Signaling Pathway and the Treatment of Psoriasis. J Immunol. 2018 Sep 15;201(6):1605-1613.
Subcellular Location
Secreted.
UNIPROT
Synonyms
IL 23 A antibody
IL 23 antibody
IL 23 subunit alpha antibody
IL 23A antibody
IL 23p19 antibody
IL-23 subunit alpha antibody
IL-23-A antibody
IL-23p19 antibody
IL12B antibody
IL23 antibody
ExpandImages
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Application: IF-Tissue
Species: Human
Site: lymph nodes
Sample: Paraffin-embedded section
Antibody concentration: 1/200 -
Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-IL-23 antibody (HA721037) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721037) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of IL-23 on different lysates with Rabbit anti-IL-23 antibody (HA721037) at 1/5,000 dilution.
Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 53 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721037, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 21 kDa
Observed band size: 21 kDa -
Western blot analysis of IL-23 on different lysates with Rabbit anti-IL-23 antibody (HA721037) at 1/5,000 dilution.
Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 53 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721037, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 21 kDa
Observed band size: 21 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"