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IL-23 Recombinant Rabbit Monoclonal Antibody [JE38-99]

Usd: 385

Specification

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Catalog# HA721037

IL-23 Recombinant Rabbit Monoclonal Antibody [JE38-99]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

IL-23 Recombinant Rabbit Monoclonal Antibody [JE38-99]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human IL23 100-189.

Species Reactivity

Human

Validated Applications

WB, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 21 kDa

Positive Control

Daudi cell lysates, THP-1 cell lysate, Raji cell lysate, human lymph nodes tissue.

Conjugation

unconjugated

Clone Number

JE38-99

RRID

AB_3072161

Reactivity Data

WB IHC-P IF-Tissue
Human
Tested Tested
Tested Tested
Tested Tested

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:400

  • IF-Tissue

  • 1:200

Target

Function

This gene encodes a subunit of the heterodimeric cytokine interleukin 23 (IL23). IL23 is composed of this protein and the p40 subunit of interleukin 12 (IL12B). The receptor of IL23 is formed by the beta 1 subunit of IL12 (IL12RB1) and an IL23 specific subunit, IL23R. Both IL23 and IL12 can activate the transcription activator STAT4, and stimulate the production of interferon-gamma (IFNG). In contrast to IL12, which acts mainly on naive CD4(+) T cells, IL23 preferentially acts on memory CD4(+) T cells. Associates with IL12B to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Background References

1. Moschen AR, et al. IL-12, IL-23 and IL-17 in IBD: immunobiology and therapeutic targeting. Nat Rev Gastroenterol Hepatol. 2019 Mar;16(3):185-196.

2. Hawkes JE, et al. Discovery of the IL-23/IL-17 Signaling Pathway and the Treatment of Psoriasis. J Immunol. 2018 Sep 15;201(6):1605-1613.

Subcellular Location

Secreted.

UNIPROT

SwissProt: Q9NPF7 Human

Synonyms

IL 23 A antibody

IL 23 antibody

IL 23 subunit alpha antibody

IL 23A antibody

IL 23p19 antibody

IL-23 subunit alpha antibody

IL-23-A antibody

IL-23p19 antibody

IL12B antibody

IL23 antibody

Expand

Images

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: lymph nodes<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/200

    Application: IF-Tissue

    Species: Human

    Site: lymph nodes

    Sample: Paraffin-embedded section

    Antibody concentration: 1/200

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-IL-23 antibody (<a href="/products/HA721037" style="font-weight: bold;text-decoration: underline;">HA721037</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721037" style="font-weight: bold;text-decoration: underline;">HA721037</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-IL-23 antibody (HA721037) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721037) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of IL-23 on different lysates with Rabbit anti-IL-23 antibody (<a href="/products/HA721037" style="font-weight: bold;text-decoration: underline;">HA721037</a>) at 1/5,000 dilution.<br /><br />Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 53 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721037" style="font-weight: bold;text-decoration: underline;">HA721037</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa

    Western blot analysis of IL-23 on different lysates with Rabbit anti-IL-23 antibody (HA721037) at 1/5,000 dilution.

    Lane 1: Raji (Human Burkitt's lymphoma cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 53 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721037, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

  • Western blot analysis of IL-23 on different lysates with Rabbit anti-IL-23 antibody (<a href="/products/HA721037" style="font-weight: bold;text-decoration: underline;">HA721037</a>) at 1/5,000 dilution.<br /><br />Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 53 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721037" style="font-weight: bold;text-decoration: underline;">HA721037</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 21 kDa<br />Observed band size: 21 kDa

    Western blot analysis of IL-23 on different lysates with Rabbit anti-IL-23 antibody (HA721037) at 1/5,000 dilution.

    Lane 1: THP-1 (Human acute monoblastic leukemia cell) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 53 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721037, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 21 kDa
    Observed band size: 21 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"