Oncostatin M Recombinant Rabbit Monoclonal Antibody [JE40-04]
Catalog# HA721038
Oncostatin M Recombinant Rabbit Monoclonal Antibody [JE40-04]
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WB
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IHC-P
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Human
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Mouse
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unconjugated
Overview
Product Name
Oncostatin M Recombinant Rabbit Monoclonal Antibody [JE40-04]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Oncostatin M aa 26-150.
Species Reactivity
Human, Mouse
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 28 kDa
Positive Control
A549 cell lysate, MCF-7 cell lysate, Hela cell lysate, mouse kidney tissue lysate, human colon carcinoma tissue.
Conjugation
unconjugated
Clone Number
JE40-04
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500
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IHC-P
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1:100
Target
Function
This gene encodes a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells. This gene and the related gene, leukemia inhibitory factor, also present on chromosome 22, may have resulted from the duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.
Background References
1. Hermanns HM. Oncostatin M and interleukin-31: Cytokines, receptors, signal transduction and physiology. Cytokine Growth Factor Rev. 2015 Oct;26(5):545-58.
2. West NR, et al. Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease. Nat Med. 2017 May;23(5):579-589.
Subcellular Location
Secreted.
Synonyms
MGC20461 antibody
ONCM_HUMAN antibody
Oncostatin M antibody
Oncostatin-M antibody
OSM antibody
Images
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Western blot analysis of Oncostatin M on different lysates with Rabbit anti-Oncostatin M antibody (HA721038) at 1/500 dilution.
Lane 1: A549 cell lysate
Lane 2: MCF-7 cell lysate
Lane 3: Hela cell lysate
Lane 4: Mouse kidney tissue lysate (20 µg/Lane)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 28 kDa
Observed band size: 22 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721038) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-Oncostatin M antibody (HA721038) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721038) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"