ITPK1 Recombinant Rabbit Monoclonal Antibody [JE64-35]
Catalog# HA721039
ITPK1 Recombinant Rabbit Monoclonal Antibody [JE64-35]
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WB
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IHC-P
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Human
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unconjugated
Overview
Product Name
ITPK1 Recombinant Rabbit Monoclonal Antibody [JE64-35]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ITPK1 aa 362-410.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
Hela cell lysate, HepG2 cell lysate, 293T cell lysate, A431 cell lysate, MCF-7 cell lysate, human liver tissue.
Conjugation
unconjugated
Clone Number
JE64-35
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
-
1:5,000
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IHC-P
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1:100
Target
Function
This gene encodes an enzyme that belongs to the inositol 1,3,4-trisphosphate 5/6-kinase family. This enzyme regulates the synthesis of inositol tetraphosphate, and downstream products, inositol pentakisphosphate and inositol hexakisphosphate. Inositol metabolism plays a role in the development of the neural tube. Disruptions in this gene are thought to be associated with neural tube defects. A pseudogene of this gene has been identified on chromosome X. Plays an important role in MLKL-mediated necroptosis. Produces highly phosphorylated inositol phosphates such as inositolhexakisphosphate (InsP6) which bind to MLKL mediating the release of an N-terminal auto-inhibitory region leading to its activation. Essential for activated phospho-MLKL to oligomerize and localize to the cell membrane during necroptosis.
Background References
1. Laha D, et al. Arabidopsis ITPK1 and ITPK2 Have an Evolutionarily Conserved Phytic Acid Kinase Activity. ACS Chem Biol. 2019 Oct 18;14(10):2127-2133.
2. Desfougères Y, et al. ITPK1 mediates the lipid-independent synthesis of inositol phosphates controlled by metabolism. Proc Natl Acad Sci U S A. 2019 Dec 3;116(49):24551-24561.
Subcellular Location
Cytosol, apical plasma membrane, cytoplasm.
UNIPROT
Synonyms
3 antibody
4)P(3) 5/6-kinase antibody
4-trisphosphate 5/6-kinase antibody
Inositol 1 3 4 triphosphate 5/6 kinase antibody
Inositol 1 3 4 trisphosphate 5/6 kinase antibody
Inositol 1 antibody
Inositol tetrakisphosphate 1 kinase antibody
Inositol triphosphate 5/6 kinase antibody
Inositol-tetrakisphosphate 1-kinase antibody
Inositol-triphosphate 5/6-kinase antibody
ExpandImages
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Western blot analysis of ITPK1 on different lysates with Rabbit anti-ITPK1 antibody (HA721039) at 1/5,000 dilution.
Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
Lane 2: Hep G2 (Human hepatocellular carcinoma cell) cell lysate
Lane 3: SK-MEL-28 (Human malignant melanoma cell) cell lysate
Lane 4: MCF7 (Human breast cancer cell) cell lysate
Lane 5: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 30 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721039, 1/5,000 in in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 46 kDa
Observed band size: 42 kDa -
Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-ITPK1 antibody (HA721039) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721039) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"