KIF5B Recombinant Rabbit Monoclonal Antibody [JE64-16] (HA721045)
Catalog# HA721045
Rabbit Monoclonal to KIF5B
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate, 10 µg/Lane
Lane 2: A549 cell lysate, 10 µg/Lane
Lane 3: Mouse kidney tissue lysate, 20 µg/Lane
Predicted band size: 110 kDa
Observed band size: 110 kDa
Exposure time: 1 minute;
8 % SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody KIF5B at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
KIF5B Recombinant Rabbit Monoclonal Antibody [JE64-16] (HA721045)
Immunogen
Synthetic peptide within human kif5b aa 1-50.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, mouse kidney tissue lysate, HCT116 cell lysate, C2C12 cell lysate, MCF-7 cell lysate, Hela cell lysate, HepG2 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, human gallbladder tissue, human testis tissue, rat uterus tissue, rat cerebellum tissue lysate, rat bladder tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE64-16
RRID
APPLICATION DILUTION
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WB
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1:500
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IHC-P
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1:100-1:400
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 110 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
KIF5B
SYNONYMS
Conventional kinesin heavy chain antibody;KIF 5B antibody;KIF5B antibody;Kinesin 1 antibody;kinesin 1 (110-120kD) antibody;Kinesin 1 heavy chain antibody;Kinesin family member 5B antibody;Kinesin heavy chain antibody;kinesin, heavy chain, ubiquitous antibody;Kinesin-1 heavy chain antibody;Kinesin1 antibody;KINH antibody;KINH_HUMAN antibody;KNS 1 antibody;KNS antibody;KNS1 antibody;Ubiquitous kinesin heavy chain antibody;UKHC antibody
SUBCELLULAR LOCATION
Cytolytic granule membrane, Lysosome membrane, cytoskeleton.
FUNCTION
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes. Can induce formation of neurite-like membrane protrusions in non-neuronal cells in a ZFYVE27-dependent manner. Regulates centrosome and nuclear positioning during mitotic entry. During the G2 phase of the cell cycle in a BICD2-dependent manner, antagonizes dynein function and drives the separation of nuclei and centrosomes. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation. Through binding with PLEKHM2 and ARL8B, directs lysosome movement toward microtubule plus ends. Involved in NK cell-mediated cytotoxicity. Drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells
CITATIONS
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Xie Jiaojiao,et al
Fibroblast growth factor 21 enhances learning and memory performance in mice by regulating hippocampal L-lactate homeostasis
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WB
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Mouse
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Citation
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