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ACTR1B Recombinant Rabbit Monoclonal Antibody [JE65-44]

Usd: 385

Specification

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Catalog# HA721050

ACTR1B Recombinant Rabbit Monoclonal Antibody [JE65-44]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

ACTR1B Recombinant Rabbit Monoclonal Antibody [JE65-44]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human ACTR1B aa 277-376/376.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 42 kDa

Positive Control

HepG2 cell lysates, Jurkat cell lysate, Hela cell lysate, human liver tissue lysate, human lung tissue lysate, human colon carcinoma tissue, human ovarian carcinoma tissue, HepG2.

Conjugation

unconjugated

Clone Number

JE65-44

RRID

AB_3072174

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:400

  • FC

  • 1:500-1:1,000

Target

Function

This gene encodes a 42.3 kD subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein and is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like ACTR1A, is an actin-related protein. These two proteins, which are of equal length and share 90% amino acid identity, are present in a constant ratio of approximately 1:15 in the dynactin complex.

Background References

1. Auzair LB. et. al. Caveolin 1 (Cav-1) and actin-related protein 2/3 complex, subunit 1B (ARPC1B) expressions as prognostic indicators for oral squamous cell carcinoma (OSCC). Eur Arch Otorhinolaryngol. 2016 Jul

2. Kumar A. et. al. Actin related protein complex subunit 1b controls sperm release, barrier integrity and cell division during adult rat spermatogenesis. Biochim Biophys Acta. 2016 Aug

Subcellular Location

Cytoskeleton, centrosome.

UNIPROT

SwissProt: P42025 Human

Synonyms

Actin related protein 1B antibody

Actin-related protein 1B antibody

ACTR 1B antibody

ACTR1B antibody

ACTY_HUMAN antibody

ARP 1B antibody

ARP1 actin related protein 1 homolog B antibody

ARP1 actin related protein 1 homolog B centractin beta (yeast) antibody

ARP1 actin related protein 1 homolog B centractin beta antibody

ARP1 actin related protein 1 yeast homolog B centractin beta antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ACTR1B antibody (<a href="/products/HA721050" style="font-weight: bold;text-decoration: underline;">HA721050</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721050" style="font-weight: bold;text-decoration: underline;">HA721050</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ACTR1B antibody (HA721050) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721050) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Rabbit anti-ACTR1B antibody (<a href="/products/HA721050" style="font-weight: bold;text-decoration: underline;">HA721050</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721050" style="font-weight: bold;text-decoration: underline;">HA721050</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue with Rabbit anti-ACTR1B antibody (HA721050) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721050) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of HepG2 cells labeling ACTR1B.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721050" style="font-weight: bold;text-decoration: underline;">HA721050</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor&reg; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling ACTR1B.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721050, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Western blot analysis of ACTR1B on different lysates with Rabbit anti-ACTR1B antibody (<a href="/products/HA721050" style="font-weight: bold;text-decoration: underline;">HA721050</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2(Human liver cancer cellsl)cell lysate<br />Lane 2: Jurkat(Human T-lymphocyte leukemia cells)cell lysate<br />Lane 3: HL-60 (Human early lymphoblastic leukemia cells) cell lysate<br />Lane 4: SK-OV-3(Human ovarian adenocarcinoma cells) cell lysate<br /> <br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721050" style="font-weight: bold;text-decoration: underline;">HA721050</a>, 1/2,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 42.3 kDa<br />Observed band size: 42 kDa

    Western blot analysis of ACTR1B on different lysates with Rabbit anti-ACTR1B antibody (HA721050) at 1/2,000 dilution.

    Lane 1: HepG2(Human liver cancer cellsl)cell lysate
    Lane 2: Jurkat(Human T-lymphocyte leukemia cells)cell lysate
    Lane 3: HL-60 (Human early lymphoblastic leukemia cells) cell lysate
    Lane 4: SK-OV-3(Human ovarian adenocarcinoma cells) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721050, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 42.3 kDa
    Observed band size: 42 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"