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MYO19 Recombinant Rabbit Monoclonal Antibody [JE64-30]

Usd: 385

Specification

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Catalog# HA721051

MYO19 Recombinant Rabbit Monoclonal Antibody [JE64-30]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

MYO19 Recombinant Rabbit Monoclonal Antibody [JE64-30]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human MYO19 aa 431-680/970.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 109 kDa

Positive Control

NCI-H441, RAW264.7 cell lysate, NIH/3T3 cell lysate, C6 cell lysate.

Conjugation

unconjugated

Clone Number

JE64-30

RRID

AB_3072175

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IF-Cell

  • 1:50

Target

Function

Myo19 encodes a novel metazoan myosin. Myo19 is expressed in multiple tissues and cell lines. Myo19 localizes to mitochondria. Expressing GFP-Myo19 alters mitochondrial dynamics. Myo19 is a novel mitochondrial associated myosin that is likely to function in actin-based mitochondrial dynamics. Actin-based motor molecule with ATPase activity that localizes to the mitochondrion outer membrane. Motor protein that moves towards the plus-end of actin filaments (By similarity). Required for mitochondrial inheritance during mitosis. May be involved in mitochondrial transport or positioning.

Background References

1. Quintero O.A. et. al. Human Myo19 is a novel myosin that associates with mitochondria. Curr. Biol. 19:2008-2013(2009)

2. Adikes R.C. et. al. Biochemical and bioinformatic analysis of the myosin-XIX motor domain. Cytoskeleton 70:281-295(2013)

Subcellular Location

Cytoskeleton, Mitochondrion outer membrane.

UNIPROT

SwissProt: Q96H55 Human

Synonyms

FLJ22865 antibody

myo19 antibody

MYO19_HUMAN antibody

Myohd1 antibody

Myosin head domain containing 1 antibody

Myosin head domain-containing protein 1 antibody

myosin XIX antibody

Myosin-XIX antibody

unconventional myosin-XIX antibody

Images

  • Immunocytochemistry analysis of NCI-H441 cells labeling MYO19 with Rabbit anti-MYO19 antibody (<a href="/products/HA721051" style="font-weight: bold;text-decoration: underline;">HA721051</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MYO19 antibody (<a href="/products/HA721051" style="font-weight: bold;text-decoration: underline;">HA721051</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor&reg; 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 647, <a href="/products/HA1127" style="font-weight: bold;text-decoration: underline;">HA1127</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NCI-H441 cells labeling MYO19 with Rabbit anti-MYO19 antibody (HA721051) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-MYO19 antibody (HA721051) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

  • Western blot analysis of MYO19 on different lysates with Rabbit anti-MYO19 antibody (<a href="/products/HA721051" style="font-weight: bold;text-decoration: underline;">HA721051</a>) at 1/5,000 dilution.<br /><br />Lane 1: RAW264.7 cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: C6 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 59 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721051" style="font-weight: bold;text-decoration: underline;">HA721051</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 109 kDa<br />Observed band size: 109 kDa

    Western blot analysis of MYO19 on different lysates with Rabbit anti-MYO19 antibody (HA721051) at 1/5,000 dilution.

    Lane 1: RAW264.7 cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: C6 cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 59 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721051, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 109 kDa
    Observed band size: 109 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"