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AAMP Recombinant Rabbit Monoclonal Antibody [JE64-70]

Usd: 385

Specification

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Catalog# HA721054

AAMP Recombinant Rabbit Monoclonal Antibody [JE64-70]

Application

  • WB

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

AAMP Recombinant Rabbit Monoclonal Antibody [JE64-70]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human AAMP aa 2-51/434.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, FC

Molecular Weight

Predicted band size: 47 kDa

Positive Control

HepG2 cell lysate, 293T cell lysate, rat testis tissue lysate, MCF-7 cell lysate, HUVEC cell lysate, NIH/3T3 cell lysate, rat brain tissue lysate, HepG2.

Conjugation

unconjugated

Clone Number

JE64-70

RRID

AB_3072178

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:2,000

  • FC

  • 1:500-1:1,000

Target

Function

Angio-associated, migratory cell protein, also known as AAMP, is a protein which in humans is encoded by the AAMP gene. This protein has been conserved in evolution and is so common to many mammals. It is found to be expressed strongly in the cytosol of endothelial cells, cytotrophoblasts, and poorly differentiated colon adenocarcinoma cells found in lymphatics and has been observed at the luminal edges of endometrial cells and in the extracellular environment of vascular-associated mesenchymal cells. AAMP helps to regulate vascular endothelial cell migration regulation and angiogenesis, with other signaling pathway like RhoA/Rho-kinase signaling. In all these diseases we can observe the expression of the AAMP gene. This one can either remain stable, increase or decrease depending on the disease. List of the diseases : gastrointestinal stromal tumor (GIST) (for this disease and the ductal carcinomas, the expression levels are to correlate with necrosis in situ), myeloid leukemia (chronic (CML) and acute (AML) forms), lymphoma, breast cancer, glial brain tumors, colon neoplasia, epidermoid carcinoma, cervical cancer, ovarian cancer, papillary thyroid cancer, pulmonary cancer, atherosclerosis, restenosis.

Background References

1. Yao S. et. al. Angio-associated migratory cell protein (AAMP) interacts with cell division cycle 42 (CDC42) and enhances migration and invasion in human non-small cell lung cancer cells. Cancer Lett. 2021 Apr

2. Wu Y. et. al. AAMP promotes colorectal cancermetastasis by suppressing SMURF2-mediatedubiquitination and degradation of RhoA. Mol Ther Oncolytics. 2021 Nov

Subcellular Location

Cell membrane, Cytoplasm.

UNIPROT

SwissProt: Q13685 Human

Entrez Gene: 227290 Mouse

Entrez Gene: 301512 Rat

Synonyms

AAMP antibody

Angio associated migratory cell protein antibody

Images

  • Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (<a href="/products/HA721054" style="font-weight: bold;text-decoration: underline;">HA721054</a>) at 1/2,000 dilution.<br /><br />Lane 1: HepG2 cell lysate, 10 µg/Lane<br />Lane 2: 293T cell lysate, 10 µg/Lane<br />Lane 3: Rat testis tissue lysate, 20 µg/Lane<br /><br />Predicted band size: 47 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 1 minute;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721054" style="font-weight: bold;text-decoration: underline;">HA721054</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/2,000 dilution.

    Lane 1: HepG2 cell lysate, 10 µg/Lane
    Lane 2: 293T cell lysate, 10 µg/Lane
    Lane 3: Rat testis tissue lysate, 20 µg/Lane

    Predicted band size: 47 kDa
    Observed band size: 50 kDa

    Exposure time: 1 minute;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (<a href="/products/HA721054" style="font-weight: bold;text-decoration: underline;">HA721054</a>) at 1/500 dilution.<br /><br />Lane 1: MCF-7 cell lysate, 10 µg/Lane<br />Lane 2: HUVEC cell lysate, 10 µg/Lane<br />Lane 3: NIH/3T3 cell lysate, 10 µg/Lane<br />Lane 4: Rat brain tissue lysate, 20 µg/Lane<br /><br />Predicted band size: 47 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721054" style="font-weight: bold;text-decoration: underline;">HA721054</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of AAMP on different lysates with Rabbit anti-AAMP antibody (HA721054) at 1/500 dilution.

    Lane 1: MCF-7 cell lysate, 10 µg/Lane
    Lane 2: HUVEC cell lysate, 10 µg/Lane
    Lane 3: NIH/3T3 cell lysate, 10 µg/Lane
    Lane 4: Rat brain tissue lysate, 20 µg/Lane

    Predicted band size: 47 kDa
    Observed band size: 50 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721054) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Flow cytometric analysis of HepG2 cells labeling AAMP.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721054" style="font-weight: bold;text-decoration: underline;">HA721054</a>, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor&reg; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HepG2 cells labeling AAMP.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721054, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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