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Septin 8 Recombinant Rabbit Monoclonal Antibody [JE64-99]

Usd: 385

Specification

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Catalog# HA721055

Septin 8 Recombinant Rabbit Monoclonal Antibody [JE64-99]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Septin 8 Recombinant Rabbit Monoclonal Antibody [JE64-99]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human Septin 8 aa 41-90/483.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 56 kDa

Positive Control

K562 cell lysate, HCT 116 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysates, mouse hippocampus tissue, mouse brain tissue, rat cerebellum tissue, rat brain tissue.

Conjugation

unconjugated

Clone Number

JE64-99

RRID

AB_3072179

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200-1:500

Target

Function

This gene is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse, and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking.

Background References

1. Farkašovský M. Septin architecture and function in budding yeast. Biol Chem. 2020 Jul

2. Falk J. et. al. Septin functions during neuro-development, a yeast perspective. Curr Opin Neurobiol. 2019 Aug

Subcellular Location

Cytoskeleton, cytoplasm, synapse, axon, synaptic vesicle membrane, presynapse.

UNIPROT

SwissProt: Q92599 Human

SwissProt: Q8CHH9 Mouse

SwissProt: B0BNF1 Rat

Synonyms

KIAA0202 antibody

OTTHUMP00000211755 antibody

SEP 2 antibody

SEP2 antibody

SEPT 8 antibody

SEPT8 antibody

Septin8 antibody

Images

  • Western blot analysis of Septin 8 on different lysates with Rabbit anti-Septin 8 antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/5,000 dilution.<br /><br />Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate<br />Lane 2: HCT 116 (Human colon cancer cell) cell lysate<br />Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate<br />Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 56 kDa<br />Observed band size: 51 kDa

    Western blot analysis of Septin 8 on different lysates with Rabbit anti-Septin 8 antibody (HA721055) at 1/5,000 dilution.

    Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
    Lane 2: HCT 116 (Human colon cancer cell) cell lysate
    Lane 3: NIH/3T3 (Mouse fibroblast) cell lysate
    Lane 4: PC-12 (Rat pheochromocytoma cell (undifferentiated)) cell lysate

    Lysates/proteins at 20 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721055, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 56 kDa
    Observed band size: 51 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Septin 8 antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-Septin 8 antibody (HA721055) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721055) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Septin 8 antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Septin 8 antibody (HA721055) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721055) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Septin 8 antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rabbit anti-Septin 8 antibody (HA721055) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721055) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Septin 8 antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721055" style="font-weight: bold;text-decoration: underline;">HA721055</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-Septin 8 antibody (HA721055) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721055) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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