PRPF4 Recombinant Rabbit Monoclonal Antibody [JE65-37] (HA721063)
Catalog# HA721063
Rabbit Monoclonal to PRPF4
Tested for
-
WB
-
IF-Cell
-
IHC-P
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PRPF4 on different lysates with Rabbit anti-PRPF4 antibody (HA721063) at 1/500 dilution.
Lane 1: HT-29 cell lysate
Lane 2: SW480 cell lysate
Lane 3: MCF-7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721063) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: HT-29 cell lysate
Lane 2: SW480 cell lysate
Lane 3: MCF-7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721063) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
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