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Adiponectin Recombinant Rabbit Monoclonal Antibody [JE65-49]

Usd: 385

Specification

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Catalog# HA721064

Adiponectin Recombinant Rabbit Monoclonal Antibody [JE65-49]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Adiponectin Recombinant Rabbit Monoclonal Antibody [JE65-49]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse Adiponectin aa 1-247/247.

Species Reactivity

Mouse

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 27 kDa

Positive Control

Mouse white adipose lysate, Mouse brown adipose tissue lysate, Mouse serum lysate, Mouse plasma lysate, NCI-H441, mouse colon tissue.

Conjugation

unconjugated

Clone Number

JE65-49

RRID

AB_3072188

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IF-Cell

  • 1:50

  • IHC-P

  • 1:1,000

Target

Function

Adiponectin (also referred to as GBP-28, apM1, AdipoQ and Acrp30) is a protein hormone and adipokine, which is involved in regulating glucose levels as well as fatty acid breakdown. In humans it is encoded by the ADIPOQ gene and it is produced in primarily in adipose tissue, but also in muscle, and even in the brain. Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation. Transgenic mice with increased adiponectin show reduced adipocyte differentiation and increased energy expenditure associated with mitochondrial uncoupling. Adiponectin is secreted into the bloodstream where it accounts for approximately 0.01% of all plasma protein at around 5-10 μg/mL (mg/L). Adiponectin automatically self-associates into larger structures. Initially, three adiponectin molecules bind together to form a homotrimer. Adiponectin exerts some of its weight reduction effects via the brain. This is similar to the action of leptin; adiponectin and leptin can act synergistically. Adiponectin promoted synaptic and memory function in the brain. Humans with lower levels of adiponectin have reduced cognitive function.

Background References

1. Choi HM. et. al. Multifaceted Physiological Roles of Adiponectin in Inflammation and Diseases. Int J Mol Sci. 2020 Feb

2. Fang H. et. al. Adiponectin Regulation and Function. Compr Physiol. 2018 Jun

Subcellular Location

Secreted.

UNIPROT

SwissProt: Q60994 Mouse

Synonyms

30 kDa adipocyte complement related protein antibody

30 kDa adipocyte complement-related protein antibody

ACDC antibody

Acrp 30 antibody

ACRP30 antibody

ADIPO_HUMAN antibody

Adipocyte antibody

Adipocyte C1q and collagen domain containing protein antibody

Adipocyte complement related 30 kDa protein antibody

Adipocyte complement related protein of 30 kDa antibody

Expand

Images

  • Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (<a href="/products/HA721064" style="font-weight: bold;text-decoration: underline;">HA721064</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse white adipose lysate<br />Lane 2: Mouse brown adipose tissue lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721064" style="font-weight: bold;text-decoration: underline;">HA721064</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (HA721064) at 1/2,000 dilution.

    Lane 1: Mouse white adipose lysate
    Lane 2: Mouse brown adipose tissue lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 30 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721064) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (<a href="/products/HA721064" style="font-weight: bold;text-decoration: underline;">HA721064</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse serum lysate<br />Lane 2: Mouse plasma lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 27 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 3 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721064" style="font-weight: bold;text-decoration: underline;">HA721064</a>) at 1/2,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Adiponectin on different lysates with Rabbit anti-Adiponectin antibody (HA721064) at 1/2,000 dilution.

    Lane 1: Mouse serum lysate
    Lane 2: Mouse plasma lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 27 kDa
    Observed band size: 30 kDa

    Exposure time: 3 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721064) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of NCI-H441 cells labeling Adiponectin with Rabbit anti-Adiponectin antibody (<a href="/products/HA721064" style="font-weight: bold;text-decoration: underline;">HA721064</a>) at 1/50 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Adiponectin antibody (<a href="/products/HA721064" style="font-weight: bold;text-decoration: underline;">HA721064</a>) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor&reg; 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 647, <a href="/products/HA1127" style="font-weight: bold;text-decoration: underline;">HA1127</a>) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of NCI-H441 cells labeling Adiponectin with Rabbit anti-Adiponectin antibody (HA721064) at 1/50 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Adiponectin antibody (HA721064) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Adiponectin antibody (<a href="/products/HA721064" style="font-weight: bold;text-decoration: underline;">HA721064</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721064" style="font-weight: bold;text-decoration: underline;">HA721064</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-Adiponectin antibody (HA721064) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721064) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation