TIM44 Recombinant Rabbit Monoclonal Antibody [JE65-55]
Catalog# HA721067
TIM44 Recombinant Rabbit Monoclonal Antibody [JE65-55]
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WB
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Human
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Rat
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unconjugated
Overview
Product Name
TIM44 Recombinant Rabbit Monoclonal Antibody [JE65-55]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TIM44 aa 1-280/452.
Species Reactivity
Human, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 51 kDa
Positive Control
PC-3 cell lysate, HeLa cell lysate, K-562 cell lysate, HepG2 cell lysate, LNCaP cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE65-55
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:5,000
Target
Function
This gene encodes a peripheral membrane protein associated with the mitochondrial inner membrane translocase, which functions in the import of proteins across the mitochondrial inner membrane and into the mitochondrial matrix. The encoded protein mediates binding of mitochondrial heat shock protein 70 to the translocase of inner mitochondrial membrane 23 (TIM23) complex. Expression of this gene is upregulated in kidney in a mouse model of diabetes. A mutation in this gene is associated with familial oncocytic thyroid carcinoma.
Background References
1. Yu X. et. al. HuR Promotes Ovarian Cancer Cell Proliferation by Regulating TIMM44 mRNA Stability. Cell Biochem Biophys. 2020 Dec
2. Hoshino A. et. al. The ADP/ATP translocase drives mitophagy independent of nucleotide exchange. Nature. 2019 Nov
Subcellular Location
Mitochondrion inner membrane, Mitochondrion matrix.
Synonyms
DKFZp686H05241 antibody
MIMT44 antibody
Mitochondrial inner membrane translocase antibody
TIM44 antibody
Translocase of inner mitochondrial membrane 44 antibody
Translocase of inner mitochondrial membrane 44 homolog (yeast) antibody
Images
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Western blot analysis of TIM44 on different lysates with Rabbit anti-TIM44 antibody (HA721067) at 1/5,000 dilution.
Lane 1: PC-3 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: HepG2 cell lysate
Lane 5: LNCaP cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 45 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721067) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"