ANXA7 Recombinant Rabbit Monoclonal Antibody [JE65-60]
Catalog# HA721068
ANXA7 Recombinant Rabbit Monoclonal Antibody [JE65-60]
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WB
-
IHC-P
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Human
-
unconjugated
Overview
Product Name
ANXA7 Recombinant Rabbit Monoclonal Antibody [JE65-60]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ANXA7 aa 1-200/488.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 53 kDa
Positive Control
293T cell lysate, A431 cell lysate, Hela cell lysate, HepG2 cell lysate, NIH/3T3 cell lysates, human colon carcinoma tissue, human pancreas tissue.
Conjugation
unconjugated
Clone Number
JE65-60
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:500
-
IHC-P
-
1:100-1:400
Target
Function
Annexin VII is a member of the annexin family of calcium-dependent phospholipid binding proteins.The Annexin VII gene contains 14 exons and spans approximately 34 kb of DNA. An alternatively spliced cassette exon results in two mRNA transcripts of 2.0 and 2.4 kb which are predicted to generate two protein isoforms differing in their N-terminal domain. The alternative splicing event is tissue specific and the mRNA containing the cassette exon is prevalent in brain, heart and skeletal muscle. The transcripts also differ in their 3'-non coding regions by the use of two alternative poly(A) signals. Annexin VII encodes a protein with a molecular weight of approximately 51 kDa with a unique, highly hydrophobic N-terminal domain of 167 amino acids and a conserved C-terminal region of 299 amino acids. The latter domain is composed of alternating hydrophobic and hydrophilic segments. Structural analysis of the protein suggests that Annexin VII is a membrane binding protein with diverse properties, including voltage-sensitive calcium channel activity, ion selectivity and membrane fusion.
Background References
1. Liu H. et. al. ANXA7 promotes the cell cycle, proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells by up-regulating CDC5L. Aging (Albany NY). 2020 Jun
2. Mo HQ. et. al. ANXA7 regulates trophoblast proliferation and apoptosis in preeclampsia. Am J Reprod Immunol. 2019 Dec
Subcellular Location
Endoplasmic reticulum membrane, extracellular exosome, nucleus, collagen-containing extracellular matrix, cytoplasm, membrane.
Synonyms
Annexin A7 antibody
Annexin VII antibody
Annexin-7 antibody
ANX7 antibody
ANXA7 antibody
ANXA7_HUMAN antibody
SNX antibody
Synexin antibody
Images
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☑ Knockdown (KD)
Western blot analysis of ANXA7 on different lysates with Rabbit anti-ANXA7 antibody (HA721068) at 1/1,000 dilution.
Lane 1: A549-WT cell lysate
Lane 2: A549-KD ANXA7 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721068) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ANXA7 on different lysates with Rabbit anti-ANXA7 antibody (HA721068) at 1/500 dilution.
Lane 1: 293T cell lysate
Lane 2: A431 cell lysate
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721068) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of ANXA7 on NIH/3T3 cell lysates with Rabbit anti-ANXA7 antibody (HA721068) at 1/500 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721068) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-ANXA7 antibody (HA721068) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721068) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-ANXA7 antibody (HA721068) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721068) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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