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CSDE1 Recombinant Rabbit Monoclonal Antibody [JE65-67]

Usd: 385

Specification

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Catalog# HA721071

CSDE1 Recombinant Rabbit Monoclonal Antibody [JE65-67]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

CSDE1 Recombinant Rabbit Monoclonal Antibody [JE65-67]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human CSDE1 aa 401-450/798.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 89 kDa

Positive Control

HeLa cell lysate, A431 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, PC-12.

Conjugation

unconjugated

Clone Number

JE65-67

RRID

AB_3072195

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • FC

  • 1:1,000

Target

Function

RNA-binding protein. Required for internal initiation of translation of human rhinovirus RNA. May be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain. Required for efficient formation of stress granules.

Background References

1. Guo AX. et. al. The role of CSDE1 in translational reprogramming and human diseases. Cell Commun Signal. 2020 Jan

2. Kakumani PK. et. al. CSDE1 controls gene expression through the miRNA-mediated decay machinery. Life Sci Alliance. 2020 Mar

Subcellular Location

Cytoplasm, Stress granule, P-body.

UNIPROT

SwissProt: O75534 Human

SwissProt: Q91W50 Mouse

SwissProt: P18395 Rat

Synonyms

Cold shock domain containing E1 RNA binding antibody

Cold shock domain containing protein E1 antibody

Cold shock domain-containing protein E1 antibody

Csde1 antibody

CSDE1_HUMAN antibody

D1S155E antibody

DKFZp779B0247 antibody

DKFZp779J1455 antibody

FLJ26882 antibody

N-ras upstream gene protein antibody

Expand

Images

  • Western blot analysis of CSDE1 on different lysates with Rabbit anti-CSDE1 antibody (<a href="/products/HA721071" style="font-weight: bold;text-decoration: underline;">HA721071</a>) at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: A431 cell lysate<br />Lane 3: RAW264.7 cell lysate<br />Lane 4: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 89 kDa<br />Observed band size: 89 kDa<br /><br />Exposure time: Lane 1: 13 seconds; Lane 2-4: 30 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721071" style="font-weight: bold;text-decoration: underline;">HA721071</a>) at 1/1,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CSDE1 on different lysates with Rabbit anti-CSDE1 antibody (HA721071) at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: A431 cell lysate
    Lane 3: RAW264.7 cell lysate
    Lane 4: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 89 kDa
    Observed band size: 89 kDa

    Exposure time: Lane 1: 13 seconds; Lane 2-4: 30 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721071) at 1/1,000 dilution was used in primary antibody dilution (K1803) at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of PC-12 cells labeling CSDE1 with Rabbit anti-CSDE1 antibody (<a href="/products/HA721071" style="font-weight: bold;text-decoration: underline;">HA721071</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSDE1 antibody (<a href="/products/HA721071" style="font-weight: bold;text-decoration: underline;">HA721071</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of PC-12 cells labeling CSDE1 with Rabbit anti-CSDE1 antibody (HA721071) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CSDE1 antibody (HA721071) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of PC-12 cells labeling CSDE1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721071" style="font-weight: bold;text-decoration: underline;">HA721071</a>, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of PC-12 cells labeling CSDE1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721071, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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