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ELOVL5 Recombinant Rabbit Monoclonal Antibody [JE65-70]

Usd: 385

Specification

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Catalog# HA721072

ELOVL5 Recombinant Rabbit Monoclonal Antibody [JE65-70]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

ELOVL5 Recombinant Rabbit Monoclonal Antibody [JE65-70]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human ELOVL5 aa 250-299/299.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 35 kDa

Positive Control

Mouse brain tissue lysates, rat lung tissue lysates.

Conjugation

unconjugated

Clone Number

JE65-70

RRID

AB_3072196

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:5,000

Target

Function

This gene belongs to the ELO family. It is highly expressed in the adrenal gland and testis, and encodes a multi-pass membrane protein that is localized in the endoplasmic reticulum. This protein is involved in the elongation of long-chain polyunsaturated fatty acids. Mutations in this gene have been associated with spinocerebellar ataxia-38 (SCA38). Alternatively spliced transcript variants have been found for this gene.

Background References

1. Liu C. et. al. Elovl2 But Not Elovl5 Is Essential for the Biosynthesis of Docosahexaenoic Acid (DHA) in Zebrafish: Insight from a Comparative Gene Knockout Study. Mar Biotechnol (NY). 2020 Oct

2. Li X. et. al. miR-21-3p Targets Elovl5 and Regulates Triglyceride Production in Mammary Epithelial Cells of Cow. DNA Cell Biol. 2019 Apr

Subcellular Location

Endoplasmic reticulum membrane, dendrite.

UNIPROT

SwissProt: Q9NYP7 Human

SwissProt: Q8BHI7 Mouse

SwissProt: Q920L7 Rat

Synonyms

3 keto acyl CoA synthase ELOVL5 antibody

3-keto acyl-CoA synthase Elovl5 antibody

dJ483K16.1 antibody

Elongation of very long chain fatty acids like 5 antibody

Elongation of very long chain fatty acids protein 5 antibody

ELOV5_HUMAN antibody

ELOVL 5 antibody

ELOVL family member 5 antibody

ELOVL family member 5 elongation of long chain fatty acids antibody

ELOVL family member 5, elongation of long chain fatty acids (FEN1/Elo2, SUR4/Elo3-like, yeast) antibody

Expand

Images

  • Western blot analysis of ELOVL5 on mouse brain tissue lysates with Rabbit anti-ELOVL5 antibody (<a href="/products/HA721072" style="font-weight: bold;text-decoration: underline;">HA721072</a>) at 1/5,000 dilution.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 33 kDa<br /><br />Exposure time: 46 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721072" style="font-weight: bold;text-decoration: underline;">HA721072</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ELOVL5 on mouse brain tissue lysates with Rabbit anti-ELOVL5 antibody (HA721072) at 1/5,000 dilution.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 33 kDa

    Exposure time: 46 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721072) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of ELOVL5 on rat lung tissue lysates with Rabbit anti-ELOVL5 antibody (<a href="/products/HA721072" style="font-weight: bold;text-decoration: underline;">HA721072</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 33 kDa<br /><br />Exposure time: 4 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721072" style="font-weight: bold;text-decoration: underline;">HA721072</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of ELOVL5 on rat lung tissue lysates with Rabbit anti-ELOVL5 antibody (HA721072) at 1/1,000 dilution.

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 33 kDa

    Exposure time: 4 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation