UCHL5IP Recombinant Rabbit Monoclonal Antibody [JE65-32]
Catalog# HA721077
UCHL5IP Recombinant Rabbit Monoclonal Antibody [JE65-32]
-
WB
-
IHC-P
-
Human
-
unconjugated
Overview
Product Name
UCHL5IP Recombinant Rabbit Monoclonal Antibody [JE65-32]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human UCHL5IP aa 150-300/368.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 40 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, human kidney tissue.
Conjugation
unconjugated
Clone Number
JE65-32
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:5,000
-
IHC-P
-
1:400
Target
Function
This gene encodes a subunit of the augmin complex, which regulates centrosome and mitotic spindle integrity, and is necessary for the completion of cytokinesis. The encoded protein was identified by interaction with ubiquitin C-terminal hydrolase 37. Alternative splicing results in multiple transcript variants. Key regulator of mitochondrial calcium uniporter (MCU) that senses calcium level via its EF-hand domains. MICU1 and MICU2 form a disulfide-linked heterodimer that stimulates and inhibits MCU activity, depending on the concentration of calcium. MICU1 acts both as an activator or inhibitor of mitochondrial calcium uptake. HAUS7 (HAUS Augmin Like Complex Subunit 7) is a Protein Coding gene. Diseases associated with HAUS7 include Vas Deferens, Congenital Bilateral Aplasia Of. Among its related pathways are Regulation of PLK1 Activity at G2/M Transition and Organelle biogenesis and maintenance. Gene Ontology (GO) annotations related to this gene include thioesterase binding. Contributes to mitotic spindle assembly, maintenance of centrosome integrity and completion of cytokinesis as part of the HAUS augmin-like complex.
Background References
1. Li L. et. al. A novel mutation in HAUS7 results in severe oligozoospermia in two brothers. Gene. 2018 Jan
Subcellular Location
Centrosome, spindle.
UNIPROT
Synonyms
26S proteasome-associated UCH interacting protein 1 antibody
26S proteasome-associated UCH37-interacting protein 1 antibody
Expressed-Xq28STS protein antibody
HAUS augmin-like complex subunit 7 antibody
Haus7 antibody
HAUS7_HUMAN antibody
HSXQ28ORF antibody
UCH37 interacting protein 1 antibody
UCHL5 interacting protein antibody
UCHL5-interacting protein antibody
ExpandImages
-
Western blot analysis of UCHL5IP on different lysates with Rabbit anti-UCHL5IP antibody (HA721077) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721077) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-UCHL5IP antibody (HA721077) at 1/400 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721077) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"