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SNX5 Recombinant Rabbit Monoclonal Antibody [JE64-96]

Usd: 385

Specification

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Catalog# HA721084

SNX5 Recombinant Rabbit Monoclonal Antibody [JE64-96]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SNX5 Recombinant Rabbit Monoclonal Antibody [JE64-96]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SNX5 aa 1-150/404.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 47 kDa

Positive Control

Mouse spleen tissue lysate, Rat spleen tissue lysate, Jurkat cell lysate, 293T cell lysate, Hela cell lysate, HepG2 cell lysate, human lymph nodes tissue, human thyroid carcinoma tissue, mouse kidney tissue, rat kidney tissue.

Conjugation

unconjugated

Clone Number

JE64-96

RRID

AB_3072208

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5000

  • IHC-P

  • 1:100-1:400

Target

Function

Sorting nexin-5 is a protein that in humans is encoded by the SNX5 gene. This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein is a component of the mammalian retromer complex, which facilitates cargo retrieval from endosomes to the trans-Golgi network. It has also been shown to bind to the Fanconi anemia, complementation group A protein. This gene results in two transcript variants encoding the same protein. Model organisms have been used in the study of SNX5 function.

Background References

1. Li J. et. al. SNX5 inhibits RLR-mediated antiviral signaling by targeting RIG-I-VISA signalosome. Biochem Biophys Res Commun. 2020 Feb

2. Zhou Q. et. al. Upregulation of SNX5 predicts poor prognosis and promotes hepatocellular carcinoma progression by modulating the EGFR-ERK1/2 signaling pathway. Oncogene. 2020 Mar

Subcellular Location

Cytoplasm, Endosome, Early endosome, Early endosome membrane, Cell membrane, Cytoplasmic vesicle membrane, phagocytic cup, ruffle.

UNIPROT

SwissProt: Q9Y5X3 Human

SwissProt: Q9D8U8 Mouse

SwissProt: B1H267 Rat

Synonyms

0910001N05Rik antibody

1810032P22Rik antibody

AU019504 antibody

D2Ertd52e antibody

FLJ10931 antibody

OTTHUMP00000030340 antibody

OTTHUMP00000062927 antibody

OTTMUSP00000003367 antibody

OTTMUSP00000003368 antibody

RP11-504H3.2 antibody

Expand

Images

  • Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate<br />Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate<br />Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate<br />Lane 4: C6 (Rat glioma cell) cell lysate<br />Lane 5: Mouse spleen tissue lysate<br />Lane 6: Rat spleen tissue lysate<br /><br />Lysates/proteins at 15 µg/Lane.<br />Exposure time: 180 seconds; ECL: K1801<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 47 kDa<br />Observed band size: 47 kDa

    Western blot analysis of SNX5 on different lysates with Rabbit anti-SNX5 antibody (HA721084) at 1/5,000 dilution.

    Lane 1: HeLa (Human cervical adenocarcinoma cell) cell lysate
    Lane 2: Jurkat (Human T-lymphoblastic cells) cell lysate
    Lane 3: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate
    Lane 4: C6 (Rat glioma cell) cell lysate
    Lane 5: Mouse spleen tissue lysate
    Lane 6: Rat spleen tissue lysate

    Lysates/proteins at 15 µg/Lane.
    Exposure time: 180 seconds; ECL: K1801

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721084, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-SNX5 antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-SNX5 antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SNX5 antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SNX5 antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721084" style="font-weight: bold;text-decoration: underline;">HA721084</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-SNX5 antibody (HA721084) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721084) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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