TCEA1 Recombinant Rabbit Monoclonal Antibody [JE64-90]
Catalog# HA721086
TCEA1 Recombinant Rabbit Monoclonal Antibody [JE64-90]
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WB
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IHC-P
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
TCEA1 Recombinant Rabbit Monoclonal Antibody [JE64-90]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human TCEA1 aa 201-250/301.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 34 kDa
Positive Control
K562 cell lysate, HUVEC cell lysate, Hela cell lysate, Jurkat cell lysate, mouse pancreas tissue lysates, rat pancreas tissue lysates, human lung cancer tissue, mouse colon tissue, rat colon tissue.
Conjugation
unconjugated
Clone Number
JE64-90
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:500-1:5,000
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IHC-P
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1:1,000
Target
Function
Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus.
Background References
1. Yang T. et. al. TCEA1 regulates the proliferative potential of mouse myeloid cells. Exp Cell Res. 2018 Sep
2. You S. et. al. Abnormal expression of YEATS4 associates with poor prognosis and promotes cell proliferation of hepatic carcinoma cell by regulation the TCEA1/DDX3 axis. Am J Cancer Res. 2018 Oct
Subcellular Location
Nucleus.
Synonyms
GTF2S antibody
SII antibody
TCEA antibody
Tcea1 antibody
TCEA1_HUMAN antibody
TF2S antibody
TFIIS antibody
Transcription elongation factor A (SII) 1 antibody
Transcription elongation factor A protein 1 antibody
Transcription elongation factor A, 1 antibody
ExpandImages
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Western blot analysis of TCEA1 on rat pancreas tissue lysates with Rabbit anti-TCEA1 antibody (HA721086) at 1/5,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 37 kDa
Exposure time: 1 minute 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721086) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Rabbit anti-TCEA1 antibody (HA721086) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-TCEA1 antibody (HA721086) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Rabbit anti-TCEA1 antibody (HA721086) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721086) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Western blot analysis of TCEA1 on different lysates with Rabbit anti-TCEA1 antibody (HA721086) at 1/1,000 dilution.
Lane 1: K-562 (Human chronic myelogenous leukemia cell) cell lysate
Lane 7: Mouse liver tissue lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 25 seconds; ECL: K1801
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA721086, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 34 kDa
Observed band size: 37 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"