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PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]

Usd: 385

Specification

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Catalog# HA721087

PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PNKP Recombinant Rabbit Monoclonal Antibody [JE64-85]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human PNKP aa 460-509/521.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 57 kDa

Positive Control

HepG2 cell lysate, 293 cell lysate, PC-3 cell lysate, Jurkat cell lysate, human colon carcinoma tissue.

Conjugation

unconjugated

Clone Number

JE64-85

RRID

AB_3072211

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500

  • IHC-P

  • 1:100

Target

Function

Bifunctional polynucleotide phosphatase/kinase is an enzyme that in humans is encoded by the PNKP gene. A detailed structural study of the crystallized mouse protein examined both the 5´-polynucleotide kinase and 3’-polynucleotide phosphatase activities. Additional features of the peptide sequence include a forkhead association (FHA) domain, ATP binding site and nuclear and mitochondrial localization sequences. PNKP has been shown to interact with DNA polymerase beta and XRCC1. The human gene encoding PNKP was observed to be mutated in patients with microcephaly, seizures and defects in DNA repair. A type of recessive ataxia is also associated with PNKP mutations. There are also newly characterized pathological variants of PNKP. Model organisms such as mice and Drosophila have been used to generate further insights.

Background References

1. Bermúdez-Guzmán L. et. al. Mutational survivorship bias: The case of PNKP. PLoS One. 2020 Dec

2. Mani RS. et. al. Domain analysis of PNKP-XRCC1 interactions: Influence of genetic variants of XRCC1. J Biol Chem. 2019 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q96T60 Human

Synonyms

2''(3'')-polynucleotidase antibody

2'(3')-polynucleotidase antibody

Bifunctional polynucleotide phosphatase/kinase antibody

DEM 1 antibody

DEM1 antibody

DNA 5' kinase/3' phosphatase antibody

DNA 5''-kinase/3''-phosphatase antibody

EIEE10 antibody

Homo sapiens polynucleotide kinase 3' phosphatase (PNKP) antibody

MCSZ antibody

Expand

Images

  • Western blot analysis of PNKP on different lysates with Rabbit anti-PNKP antibody (<a href="/products/HA721087" style="font-weight: bold;text-decoration: underline;">HA721087</a>) at 1/500 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: 293 cell lysate<br />Lane 3: PC-3 cell lysate<br />Lane 4: Jurkat cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 57 kDa<br />Observed band size: 60 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721087" style="font-weight: bold;text-decoration: underline;">HA721087</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PNKP on different lysates with Rabbit anti-PNKP antibody (HA721087) at 1/500 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: 293 cell lysate
    Lane 3: PC-3 cell lysate
    Lane 4: Jurkat cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 57 kDa
    Observed band size: 60 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721087) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PNKP antibody (<a href="/products/HA721087" style="font-weight: bold;text-decoration: underline;">HA721087</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721087" style="font-weight: bold;text-decoration: underline;">HA721087</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-PNKP antibody (HA721087) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721087) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"