BRAT1 Recombinant Rabbit Monoclonal Antibody [JE64-71] (HA721091)
Catalog# HA721091
Rabbit Monoclonal to BRAT1
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of BRAT1 on different lysates with Rabbit anti-BRAT1 antibody (HA721091) at 1/500 dilution.
Lane 1: MCF-7 cell lysate
Lane 2: PC-3 cell lysate
Lane 3: 293 cell lysate
Lane 4: K562 cell lysate
Lane 5: A431 cell lysate
Lane 6: THP-1 cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 77 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721091) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lane 1: MCF-7 cell lysate
Lane 2: PC-3 cell lysate
Lane 3: 293 cell lysate
Lane 4: K562 cell lysate
Lane 5: A431 cell lysate
Lane 6: THP-1 cell lysate
Lane 7: A549 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 88 kDa
Observed band size: 77 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721091) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
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