Skip to content

eIF1A Recombinant Rabbit Monoclonal Antibody [JE64-52]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA721102

eIF1A Recombinant Rabbit Monoclonal Antibody [JE64-52]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

eIF1A Recombinant Rabbit Monoclonal Antibody [JE64-52]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human eIF1A aa 71-120/144.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell

Target Molecular Weight

Predicted band size: 16 kDa

Positive Control

Jurkat cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, HepG2.

Conjugation

unconjugated

Clone Number

JE64-52

RRID

AB_3072226

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

Eukaryotic translation initiation factor 1A, X-chromosomal (eIF1A) is a protein that in humans is encoded by the EIF1AX gene. This gene encodes an essential eukaryotic translation initiation factor. The protein is a component of the 43S pre-initiation complex (PIC), which mediates the recruitment of the small 40S ribosomal subunit to the 5' cap of messenger RNAs. eIF1A is a small protein (17 kDa in budding yeast) and a component of the 43S preinitiation complexes (PIC). eIF1A binds near the ribosomal A-site, in a manner similar to the functionally related bacterial counterpart IF1. Mutations in this gene have been recurrently seen associated to cases of uveal melanoma with disomy 3. eIF1A is mutated in thyroid cancers. EIF1AX has been shown to interact with IPO13.

Background References

1. Geng R. et. al. EIF1A depletion restrains human pituitary adenoma progression. Transl Oncol. 2022 Jan

2. Yu J. et. al. Comparative sequence and structure analysis of eIF1A and eIF1AD. BMC Struct Biol. 2018 Sep

Subcellular Location

Cytosol.

UNIPROT

SwissProt: P47813 Human

Synonyms

eIF-1A antibody

eIF-1A X isoform antibody

eIF-4C antibody

EIF1A antibody

EIF1AP1 antibody

EIF1AX antibody

EIF4C antibody

eukaryotic translation initiation factor 1A X-linked antibody

eukaryotic translation initiation factor 1A, X chromosome antibody

Eukaryotic translation initiation factor 1A, X-chromosomal antibody

Expand

Images

  • Western blot analysis of eIF1A on different lysates with Rabbit anti-eIF1A antibody (<a href="/products/HA721102" style="font-weight: bold;text-decoration: underline;">HA721102</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: NIH/3T3 cell lysate<br />Lane 3: PC-12 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 16 kDa<br />Observed band size: 16 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721102" style="font-weight: bold;text-decoration: underline;">HA721102</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of eIF1A on different lysates with Rabbit anti-eIF1A antibody (HA721102) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: NIH/3T3 cell lysate
    Lane 3: PC-12 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 16 kDa
    Observed band size: 16 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721102) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HepG2 cells labeling eIF1A with Rabbit anti-eIF1A antibody (<a href="/products/HA721102" style="font-weight: bold;text-decoration: underline;">HA721102</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF1A antibody (<a href="/products/HA721102" style="font-weight: bold;text-decoration: underline;">HA721102</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HepG2 cells labeling eIF1A with Rabbit anti-eIF1A antibody (HA721102) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-eIF1A antibody (HA721102) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"