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hnRNP G-T Recombinant Rabbit Monoclonal Antibody [JE64-33]

Usd: 385

Specification

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Catalog# HA721104

hnRNP G-T Recombinant Rabbit Monoclonal Antibody [JE64-33]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

hnRNP G-T Recombinant Rabbit Monoclonal Antibody [JE64-33]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human hnRNP G-T aa 151-350/392.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, IHC-P

Molecular Weight

Predicted band size: 43 kDa

Positive Control

293 cell lysate, Hela cell lysate, rat testis tissue lysates, Hela, human testis tissue, mouse testis tissue.

Conjugation

unconjugated

Clone Number

JE64-33

RRID

AB_3072228

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:500-1:5,000

  • IF-Cell

  • 1:200

  • IHC-P

  • 1:400

Target

Function

This gene belongs to the HNRPG subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two RRM domains that bind RNAs. This gene is intronless and is thought to be derived from a processed retroposon. However, unlike many retroposon-derived genes, this gene is not a pseudogene. The encoded protein has similarity to HNRPG and RBMY proteins and it is suggested to replace HNRPG protein function during meiotic prophase or act as a germ cell-specific splicing regulator. It primarily localizes to the nuclei of meiotic spermatocytes. This gene is a candidate for autosomal male infertility.

Background References

1. Liu Y. et. al. The germ cell nuclear proteins hnRNP G-T and RBMY activate a testis-specific exon. PLoS Genet. 2009 Nov

2. Maymon BB. et. al. Localization of the germ cell-specific protein, hnRNP G-T, in testicular biopsies of azoospermic men. Acta Histochem. 2002

Subcellular Location

Nucleus.

UNIPROT

SwissProt: O75526 Human

SwissProt: Q9DAE2 Mouse

Entrez Gene: 365344 Rat

Synonyms

Heterogeneous nuclear ribonucleoprotein G T antibody

hnRNP G T antibody

hnRNP G-T antibody

HNRNPG T antibody

HNRNPGT antibody

HNRPGT antibody

RBMX L2 antibody

RBMXL 2 antibody

RBMXL2 antibody

RMXL2_HUMAN antibody

Expand

Images

  • Western blot analysis of hnRNP G-T on different lysates with Rabbit anti-hnRNP G-T antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/5,000 dilution.<br /><br />Lane 1: 293 cell lysate<br />Lane 2: Hela cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 2 minutes;<br /><br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of hnRNP G-T on different lysates with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/5,000 dilution.

    Lane 1: 293 cell lysate
    Lane 2: Hela cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 2 minutes;

    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721104) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of hnRNP G-T on rat testis tissue lysates with Rabbit anti-hnRNP G-T antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 43 kDa<br />Observed band size: 43 kDa<br /><br />Exposure time: 2 minutes;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:200,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of hnRNP G-T on rat testis tissue lysates with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 43 kDa
    Observed band size: 43 kDa

    Exposure time: 2 minutes;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721104) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Hela cells labeling hnRNP G-T with Rabbit anti-hnRNP G-T antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/200 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-hnRNP G-T antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor&reg; 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta Ⅲ tubulin (<a href="/products/M0805-8" style="font-weight: bold;text-decoration: underline;">M0805-8</a>, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor&reg; 647) were used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Hela cells labeling hnRNP G-T with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/200 dilution.

    Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

    Beta Ⅲ tubulin (M0805-8, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) were used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-hnRNP G-T antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721104) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-hnRNP G-T antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721104" style="font-weight: bold;text-decoration: underline;">HA721104</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-hnRNP G-T antibody (HA721104) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721104) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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