PTPRR / PCPTP1 Recombinant Rabbit Monoclonal Antibody [JE64-84]
Catalog# HA721118
PTPRR / PCPTP1 Recombinant Rabbit Monoclonal Antibody [JE64-84]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
PTPRR / PCPTP1 Recombinant Rabbit Monoclonal Antibody [JE64-84]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human PTPRR aa 1-50/657.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 74 kDa
Positive Control
HeLa cell lysate, SH-SY5Y cell lysate, Rat brain tissue lysates.
Conjugation
unconjugated
Clone Number
JE64-84
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:5,000
Target
Function
The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. Silencing of this gene has been associated with colorectal cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares a symbol (PTPRQ) with another gene, protein tyrosine phosphatase, receptor type, Q (GeneID 374462), which is also located on chromosome 12.
Background References
1. Wang JL. et. al. Identification of PTPRR and JAG1 as key genes in castration-resistant prostate cancer by integrated bioinformatics methods. J Zhejiang Univ Sci B. 2020 Mar
2. Wang Y. et. al. Protein tyrosine phosphatase receptor type R (PTPRR) antagonizes the Wnt signaling pathway in ovarian cancer by dephosphorylating and inactivating beta-catenin. J Biol Chem. 2019 Nov
Subcellular Location
Cell membrane; Perinuclear region.
Synonyms
Ch-1PTPase antibody
Ch1 PTPase antibody
DKFZp781C1038 antibody
EC PTP antibody
ECPTP antibody
FLJ34328 antibody
MGC131968 antibody
MGC148170 antibody
NC PTPCOM1 antibody
NC-PTPCOM1 antibody
ExpandImages
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Western blot analysis of PTPRR / PCPTP1 on different lysates with Rabbit anti-PTPRR / PCPTP1 antibody (HA721118) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: SH-SY5Y cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 70 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721118) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Western blot analysis of PTPRR / PCPTP1 on Rat brain tissue lysates with Rabbit anti-PTPRR / PCPTP1 antibody (HA721118) at 1/500 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721118) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"