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PTPRR / PCPTP1 Recombinant Rabbit Monoclonal Antibody [JE64-84]

Usd: 385

Specification

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Catalog# HA721118

PTPRR / PCPTP1 Recombinant Rabbit Monoclonal Antibody [JE64-84]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

PTPRR / PCPTP1 Recombinant Rabbit Monoclonal Antibody [JE64-84]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human PTPRR aa 1-50/657.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 74 kDa

Positive Control

HeLa cell lysate, SH-SY5Y cell lysate, Rat brain tissue lysates.

Conjugation

unconjugated

Clone Number

JE64-84

RRID

AB_3072242

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

Target

Function

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and a single intracellular catalytic domain, and thus represents a receptor-type PTP. Silencing of this gene has been associated with colorectal cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares a symbol (PTPRQ) with another gene, protein tyrosine phosphatase, receptor type, Q (GeneID 374462), which is also located on chromosome 12.

Background References

1. Wang JL. et. al. Identification of PTPRR and JAG1 as key genes in castration-resistant prostate cancer by integrated bioinformatics methods. J Zhejiang Univ Sci B. 2020 Mar

2. Wang Y. et. al. Protein tyrosine phosphatase receptor type R (PTPRR) antagonizes the Wnt signaling pathway in ovarian cancer by dephosphorylating and inactivating beta-catenin. J Biol Chem. 2019 Nov

Subcellular Location

Cell membrane; Perinuclear region.

UNIPROT

SwissProt: Q15256 Human

SwissProt: O08617 Rat

Synonyms

Ch-1PTPase antibody

Ch1 PTPase antibody

DKFZp781C1038 antibody

EC PTP antibody

ECPTP antibody

FLJ34328 antibody

MGC131968 antibody

MGC148170 antibody

NC PTPCOM1 antibody

NC-PTPCOM1 antibody

Expand

Images

  • Western blot analysis of PTPRR / PCPTP1 on different lysates with Rabbit anti-PTPRR / PCPTP1 antibody (<a href="/products/HA721118" style="font-weight: bold;text-decoration: underline;">HA721118</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: SH-SY5Y cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 70 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721118" style="font-weight: bold;text-decoration: underline;">HA721118</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PTPRR / PCPTP1 on different lysates with Rabbit anti-PTPRR / PCPTP1 antibody (HA721118) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: SH-SY5Y cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 70 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721118) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PTPRR / PCPTP1 on Rat brain tissue lysates with Rabbit anti-PTPRR / PCPTP1 antibody (<a href="/products/HA721118" style="font-weight: bold;text-decoration: underline;">HA721118</a>) at 1/500 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 74 kDa<br />Observed band size: 74 kDa<br /><br />Exposure time: 2 minutes;<br /><br />8% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721118" style="font-weight: bold;text-decoration: underline;">HA721118</a>) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PTPRR / PCPTP1 on Rat brain tissue lysates with Rabbit anti-PTPRR / PCPTP1 antibody (HA721118) at 1/500 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 74 kDa
    Observed band size: 74 kDa

    Exposure time: 2 minutes;

    8% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721118) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"