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NDUFC2 Recombinant Rabbit Monoclonal Antibody [JE65-31]

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Specification

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Catalog# HA721123

NDUFC2 Recombinant Rabbit Monoclonal Antibody [JE65-31]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

NDUFC2 Recombinant Rabbit Monoclonal Antibody [JE65-31]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human NDUFC2 aa 1-119.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 14 kDa

Positive Control

HeLa cell lysate, HepG2 cell lysate, A549 cell lysate, human kidney tissue, human liver tissue.

Conjugation

unconjugated

Clone Number

JE65-31

RRID

AB_3072247

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:5,000

  • IHC-P

  • 1:200-1:1,000

Target

Function

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. Through use of chaotropic agents, complex I can be separated into 3 different fractions: a flavoprotein fraction, an iron-sulfur protein (IP) fraction and a hydrophobic protein (HP) fraction. NDUFC2 (NADH dehydrogenase [ubiquinone] 1 subunit C2), also known as B14.5b or NADHDH2, is a 119 amino acid mitochondrion inner single-pass membrane protein that belongs to the complex I NDUFC2 subunit family. NDUFC2 is an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is not involved in catalysis. Complex I is composed of 45 different subunits and functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is suggested to be ubiquinone.

Background References

1. Raffa S. et. al. The reduction of NDUFC2 expression is associated with mitochondrial impairment in circulating mononuclear cells of patients with acute coronary syndrome. Int J Cardiol. 2019 Jul

2. Madonna R. et. al. Deficiency of NDUFC2: Cause or bystander in acute coronary syndromes? Int J Cardiol. 2019 Jul

Subcellular Location

Mitochondrion inner membrane.

UNIPROT

SwissProt: O95298 Human

Synonyms

B14.5b antibody

CI-B14.5b antibody

Complex I B14.5b antibody

Complex I subunit B14.5b antibody

Complex I-B14.5b antibody

HLC-1 antibody

HLC1 antibody

Human lung cancer oncogene 1 protein antibody

NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2, 14.5kDa antibody

NADH dehydrogenase [ubiquinone] 1 subunit C2 antibody

Expand

Images

  • Western blot analysis of NDUFC2 on different lysates with Rabbit anti-NDUFC2 antibody (<a href="/products/HA721123" style="font-weight: bold;text-decoration: underline;">HA721123</a>) at 1/5,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HepG2 cell lysate<br />Lane 3: A549 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 14 kDa<br />Observed band size: 14 kDa<br /><br />Exposure time: 10 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721123" style="font-weight: bold;text-decoration: underline;">HA721123</a>) at 1/5,000 dilution was used in primary antibody dilution (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of NDUFC2 on different lysates with Rabbit anti-NDUFC2 antibody (HA721123) at 1/5,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HepG2 cell lysate
    Lane 3: A549 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 14 kDa
    Observed band size: 14 kDa

    Exposure time: 10 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721123) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFC2 antibody (<a href="/products/HA721123" style="font-weight: bold;text-decoration: underline;">HA721123</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721123" style="font-weight: bold;text-decoration: underline;">HA721123</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-NDUFC2 antibody (HA721123) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721123) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NDUFC2 antibody (<a href="/products/HA721123" style="font-weight: bold;text-decoration: underline;">HA721123</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721123" style="font-weight: bold;text-decoration: underline;">HA721123</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-NDUFC2 antibody (HA721123) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721123) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"