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PEX11B Recombinant Rabbit Monoclonal Antibody [JE64-51]

Usd: 385

Specification

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Catalog# HA721124

PEX11B Recombinant Rabbit Monoclonal Antibody [JE64-51]

Application

  • WB

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

PEX11B Recombinant Rabbit Monoclonal Antibody [JE64-51]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human PEX11B aa 101-150/259.

Species Reactivity

Human, Mouse

Validated Applications

WB

Molecular Weight

Predicted band size: 28 kDa

Positive Control

Human lung tissue lysate, MCF-7 cell lysate.

Conjugation

unconjugated

Clone Number

JE64-51

RRID

AB_3072248

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The protein encoded by this gene facilitates peroxisomal proliferation and interacts with PEX19. The encoded protein is found in the peroxisomal membrane. Several transcript variants, some protein-coding and some not protein-coding, have been found for this gene.

Background References

1. Tian Y. et. al. Variant analysis of PEX11B gene from a family with peroxisome biogenesis disorder 14B by whole exome sequencing. Mol Genet Genomic Med. 2020 Jan

2. Lismont C. et. al. Deciphering the potential involvement of PXMP2 and PEX11B in hydrogen peroxide permeation across the peroxisomal membrane reveals a role for PEX11B in protein sorting. Biochim Biophys Acta Biomembr. 2019 Oct

Subcellular Location

Peroxisome membrane.

UNIPROT

SwissProt: O96011 Human

Synonyms

OTTHUMP00000015598 antibody

Peroxin 11B antibody

Peroxin-11B antibody

peroxisomal biogenesis factor 11 beta antibody

Peroxisomal biogenesis factor 11B antibody

Peroxisomal membrane protein 11B antibody

PEX11 beta antibody

PEX11-beta antibody

PEX11B antibody

Pex11p beta antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (<a href="/products/HA721124" style="font-weight: bold;text-decoration: underline;">HA721124</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-PEX11B KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28 kDa<br /><br />Exposure time: 40 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721124" style="font-weight: bold;text-decoration: underline;">HA721124</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (HA721124) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-PEX11B KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

    Exposure time: 40 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721124) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (<a href="/products/HA721124" style="font-weight: bold;text-decoration: underline;">HA721124</a>) at 1/5,000 dilution.<br /><br />Lane 1: MCF-7 (Human breast cancer cell) cell lysate<br />Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate<br /><br /><br />Lysates/proteins at 20 µg/Lane.<br />Exposure time: 20 seconds; ECL: K1801<br /><br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721124" style="font-weight: bold;text-decoration: underline;">HA721124</a>, 1/5,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28 kDa

    Western blot analysis of PEX11B on different lysates with Rabbit anti-PEX11B antibody (HA721124) at 1/5,000 dilution.

    Lane 1: MCF-7 (Human breast cancer cell) cell lysate
    Lane 2: RAW264.7 (Mouse monocytic macrophage leukemia cell) cell lysate


    Lysates/proteins at 20 µg/Lane.
    Exposure time: 20 seconds; ECL: K1801


    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721124, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation