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PREB Recombinant Rabbit Monoclonal Antibody [JE64-56]

Usd: 385

Specification

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Catalog# HA721125

PREB Recombinant Rabbit Monoclonal Antibody [JE64-56]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PREB Recombinant Rabbit Monoclonal Antibody [JE64-56]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human PREB aa 81-230/417.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 45 kDa

Positive Control

HepG2 cell lysates, human lymph nodes tissue, human colon tissue.

Conjugation

unconjugated

Clone Number

JE64-56

RRID

AB_3072249

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2000-1:10000

  • IHC-P

  • 1:100-1:400

Target

Function

This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. Prolactin regulatory element-binding protein is a protein that in humans is encoded by the PREB gene. This gene encodes a protein that specifically binds to a Pit1-binding element of the prolactin (PRL) promoter. This protein may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p. This gene overlaps the abhydrolase domain containing 1 (ABHD1) gene on the opposite strand.

Background References

1. Khass M. et. al. Disruption of the preB Cell Receptor Complex Leads to Decreased Bone Mass. Front Immunol. 2019 Sep

2. Park JM. et. al. Prolactin regulatory element-binding (PREB) protein regulates hepatic glucose homeostasis. Biochim Biophys Acta Mol Basis Dis. 2018 Jun

Subcellular Location

Endoplasmic reticulum membrane, Nucleus.

UNIPROT

SwissProt: Q9HCU5 Human

Synonyms

Mammalian guanine nucleotide exchange factor mSec12 antibody

Preb antibody

PREB_HUMAN antibody

Prolactin regulatory binding element protein antibody

Prolactin regulatory element binding antibody

Prolactin regulatory element binding protein antibody

Prolactin regulatory element-binding protein antibody

SEC12 antibody

Images

  • Western blot analysis of PREB on HepG2 (Human liver cancer cell) cell lysate with Rabbit anti-PREB antibody (<a href="/products/HA721125" style="font-weight: bold;text-decoration: underline;">HA721125</a>) at 1/10,000 dilution.<br /><br />Lysates/proteins at 10 µg/Lane.<br />Exposure time: 3 minutes; ECL: K1802<br /><br />Blocking: 5% NFDM/TBST, 1 hour at room temperature<br />Primary antibody: <a href="/products/HA721125" style="font-weight: bold;text-decoration: underline;">HA721125</a>, 1/10,000 in primary antibody dilution buffer (<a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a>), overnight at 4 ℃<br />Secondary antibody: Goat anti-Rabbit IgG-HRP (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature<br /><br />Predicted band size: 45.5 kDa<br />Observed band size: 45 kDa

    Western blot analysis of PREB on HepG2 (Human liver cancer cell) cell lysate with Rabbit anti-PREB antibody (HA721125) at 1/10,000 dilution.

    Lysates/proteins at 10 µg/Lane.
    Exposure time: 3 minutes; ECL: K1802

    Blocking: 5% NFDM/TBST, 1 hour at room temperature
    Primary antibody: HA721125, 1/10,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃
    Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature

    Predicted band size: 45.5 kDa
    Observed band size: 45 kDa

  • Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-PREB antibody (<a href="/products/HA721125" style="font-weight: bold;text-decoration: underline;">HA721125</a>) at 1/400 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721125" style="font-weight: bold;text-decoration: underline;">HA721125</a>) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Rabbit anti-PREB antibody (HA721125) at 1/400 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721125) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PREB antibody (<a href="/products/HA721125" style="font-weight: bold;text-decoration: underline;">HA721125</a>) at 1/100 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721125" style="font-weight: bold;text-decoration: underline;">HA721125</a>) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human colon tissue with Rabbit anti-PREB antibody (HA721125) at 1/100 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721125) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"