HRP Conjugated GAPDH Recombinant Rabbit Monoclonal Antibody [SA30-01] (HA721131)
Catalog# HA721131
Rabbit Monoclonal to GAPDH
-
WB
-
IHC
-
Human
-
Mouse
-
Rat
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
HRP Conjugated GAPDH Recombinant Rabbit Monoclonal Antibody [SA30-01] (HA721131)
Immunogen
Recombinant protein within mouse gapdh aa 94-333 / 333.
Host
Rabbit
Positive Control
HepG2 cell lysates, PC-12 cell lysates, NIH/3T3 cell lysates, THP-1 cell lysate, MCF-7 cell lysate, RH-35 cell lysate, HepG2 cell lysate, PC-12 cell lysate, L6 cell lysate, RAW264.7 cell lysate, A549 cell lysate, PMVEC cell lysate, NIH/3T3 cell lysate, B16F1 cell lysate.
Conjugation
Hrp
Clonality
Monoclonal
Clone Number
SA30-01
RRID
APPLICATION DILUTION
-
WB
-
1:2,500-1:20,000
-
IHC:1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol.
Concentration
0.5ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 36 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GAPDH
SYNONYMS
38 kDa BFA-dependent ADP-ribosylation substrate antibody;aging associated gene 9 protein antibody;Aging-associated gene 9 protein antibody;BARS-38 antibody;cb609 antibody;EC 1.2.1.12 antibody;Epididymis secretory sperm binding protein Li 162eP antibody;G3P_HUMAN antibody;G3PD antibody;G3PDH antibody;GAPD antibody;GAPDH antibody;Glyceraldehyde 3 phosphate dehydrogenase antibody;Glyceraldehyde-3-phosphate dehydrogenase antibody;HEL-S-162eP antibody;KNC-NDS6 antibody;MGC102544 antibody;MGC102546 antibody;MGC103190 antibody;MGC103191 antibody;MGC105239 antibody;MGC127711 antibody;MGC88685 antibody;OCAS, p38 component antibody;OCT1 coactivator in S phase, 38-KD component antibody;peptidyl cysteine S nitrosylase GAPDH antibody;Peptidyl-cysteine S-nitrosylase GAPDH antibody;wu:fb33a10 antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Membrane.
FUNCTION
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
CITATIONS
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