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HRP Conjugated GAPDH Recombinant Rabbit Monoclonal Antibody [SA30-01] - Loading control

Usd: 409

Specification

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Catalog# HA721131

HRP Conjugated GAPDH Recombinant Rabbit Monoclonal Antibody [SA30-01] - Loading control

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • HRP

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

HRP Conjugated GAPDH Recombinant Rabbit Monoclonal Antibody [SA30-01] - Loading control

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within mouse GAPDH aa 94-333 / 333.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 36 kDa

Positive Control

HepG2 cell lysates, PC-12 cell lysates, NIH/3T3 cell lysates, THP-1 cell lysate, MCF-7 cell lysate, RH-35 cell lysate, HepG2 cell lysate, PC-12 cell lysate, L6 cell lysate, RAW264.7 cell lysate, A549 cell lysate, PMVEC cell lysate, NIH/3T3 cell lysate, B16F1 cell lysate.

Conjugation

HRP

Clone Number

SA30-01

RRID

AB_3072255

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:10,000-1:20,000

  • IHC-P

  • 1:200-1:500

Target

Function

Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.

Background References

1. "High-resolution structure of human D-glyceraldehyde-3-phosphate dehydrogenase."Jenkins J.L., Tanner J.J.Acta Crystallogr. D 62:290-301(2006)

2. "Structural analysis of human liver glyceraldehyde-3-phosphate dehydrogenase."Ismail S.A., Park H.W.Acta Crystallogr. D 61:1508-1513(2005)

Subcellular Location

Cytoplasm, Nucleus, Membrane.

UNIPROT

SwissProt: P04406 Human

SwissProt: P16858 Mouse

SwissProt: P04797 Rat

Synonyms

38 kDa BFA-dependent ADP-ribosylation substrate antibody

aging associated gene 9 protein antibody

Aging-associated gene 9 protein antibody

BARS-38 antibody

cb609 antibody

EC 1.2.1.12 antibody

Epididymis secretory sperm binding protein Li 162eP antibody

G3P_HUMAN antibody

G3PD antibody

G3PDH antibody

Expand

Images

  • Western blot analysis of GAPDH on HepG2 cell lysates with Rabbit anti-GAPDH antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at different dilutions.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 36 kDa<br /><br />Exposure time: 30 seconds;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of GAPDH on HepG2 cell lysates with Rabbit anti-GAPDH antibody (HA721131) at different dilutions.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

    Exposure time: 30 seconds;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

  • Western blot analysis of GAPDH on PC-12 cell lysates with Rabbit anti-GAPDH antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at different dilutions.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 36 kDa<br /><br />Exposure time: 30 seconds;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of GAPDH on PC-12 cell lysates with Rabbit anti-GAPDH antibody (HA721131) at different dilutions.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

    Exposure time: 30 seconds;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GAPDH antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at 1/50 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-GAPDH antibody (HA721131) at 1/50 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721131) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Western blot analysis of GAPDH on NIH/3T3 cell lysates with Rabbit anti-GAPDH antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at different dilutions.<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 36 kDa<br /><br />Exposure time: 30 seconds;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of GAPDH on NIH/3T3 cell lysates with Rabbit anti-GAPDH antibody (HA721131) at different dilutions.

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

    Exposure time: 30 seconds;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at different dilutions were used in 5% NFDM/TBST at room temperature for 2 hours.

  • Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at 1/10,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: MCF-7 cell lysate<br />Lane 3: RH-35 cell lysate<br />Lane 4: HepG2 cell lysate<br />Lane 5: PC-12 cell lysate<br />Lane 6: L6 cell lysate<br />Lane 7: RAW264.7 cell lysate<br />Lane 8: A549 cell lysate<br />Lane 9: PMVEC cell lysate<br />Lane 10: NIH/3T3 cell lysate<br />Lane 11: B16F1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 36 kDa<br />Observed band size: 36 kDa<br /><br />Exposure time: 30 seconds;<br /><br />10% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

    Western blot analysis of GAPDH on different lysates with Rabbit anti-GAPDH antibody (HA721131) at 1/10,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: MCF-7 cell lysate
    Lane 3: RH-35 cell lysate
    Lane 4: HepG2 cell lysate
    Lane 5: PC-12 cell lysate
    Lane 6: L6 cell lysate
    Lane 7: RAW264.7 cell lysate
    Lane 8: A549 cell lysate
    Lane 9: PMVEC cell lysate
    Lane 10: NIH/3T3 cell lysate
    Lane 11: B16F1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 36 kDa
    Observed band size: 36 kDa

    Exposure time: 30 seconds;

    10% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721131) at 1/10,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours.

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GAPDH antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Rabbit anti-GAPDH antibody (HA721131) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721131) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GAPDH antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721131" style="font-weight: bold;text-decoration: underline;">HA721131</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat liver tissue with Rabbit anti-GAPDH antibody (HA721131) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721131) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

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