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Estrogen Receptor alpha Recombinant Rabbit Monoclonal Antibody [PD00-04]

Usd: 385

Specification

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Catalog# HA721140

Estrogen Receptor alpha Recombinant Rabbit Monoclonal Antibody [PD00-04]

Application

  • IHC-P

  • IF-Tissue

  • mIHC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Estrogen Receptor alpha Recombinant Rabbit Monoclonal Antibody [PD00-04]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Estrogen Receptor alpha aa 300-595 / 595.

Species Reactivity

Human

Validated Applications

IHC-P, IF-Tissue, mIHC

Molecular Weight

Predicted band size: 66 kDa

Positive Control

Human breast tissue, human cervix tissue, human breast carcinoma tissue, human endometrium tissue.

Conjugation

unconjugated

Clone Number

PD00-04

RRID

AB_3072262

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • IHC-P

  • 1:1,000-1:5,000

  • IF-Tissue

  • 1:500

  • mIHC

  • 1:1,000

Target

Function

Estrogen receptor (ER) belongs to the steroid receptor superfamily of nuclear receptors. It is a protein with 553 amino acids. ER mediates regulatory functions of female sex steroids, mainly 17 (E2), on growth, differentiation and function in several target tissues, including female and male reproductive tract, mammary gland, and skeletal and cardiovascular systems. In normal and malignant human breast tissue ER is expressed in stromal cells in addition to epithelia. Only limited data are available on the role of ER in normal and neoplastic tissues. ER is mainly expressed in tumours of female sex steroid hormone responsive tissues such as the mammary gland, endometrium, and ovary. ER protein is expressed in 60-70% of female breast cancers (ER+/PR- 19-22%; ER+/PR+ 49-53%). Other tumours expressing ER are meningiomas, salivary gland tumours, some neuroendocrine tumours, and some colorectal and hepatocellular carcinomas. Uterine cervix and tonsil can be recommended as positive tissue controls for ER. In uterine cervix, virtually all squamous and columnar epithelial cells must show a moderate to strong and distinct nuclear staining reaction. Lymphocytes and endothelial cells must be negative. Tonsil is especially found recommendable as a tool to monitor the level of analytical sensitivity for the demonstration of ER. Dispersed follicular dendritic cells in germinal centers and squamous epithelial cells must show an at least weak but distinct nuclear staining reaction. In addition, tonsil can be used as negative tissue control, as B-cells in mantle zones and within germinal centers must be negative. To validate the specificity of the IHC protocol further, an ER negative breast carcinoma must be included as primary negative tissue control, in which only remnants of normal epithelial and stromal cells should be ER positive, serving as internal positive tissue control. Positive staining reaction of the stromal cells in breast tissue indicates that the IHC protocol provides a high analytical sensitivity for ER, whereas the analytical sensitivity cannot reliably be evaluated in normal epithelial cells in breast as they typically express moderate to high levels of ER.

Background References

1. Brett JO. et. al. ESR1 mutation as an emerging clinical biomarker in metastatic hormone receptor-positive breast cancer. Breast Cancer Res. 2021 Aug

2. Turner NC. et. al. ESR1 Mutations and Overall Survival on Fulvestrant versus Exemestane in Advanced Hormone Receptor-Positive Breast Cancer: A Combined Analysis of the Phase III SoFEA and EFECT Trials. Clin Cancer Res. 2020 Oct

Subcellular Location

Cell membrane, Nucleus, Cytoplasm.

UNIPROT

SwissProt: P03372 Human

Synonyms

7*/654 isoform antibody

7*/819 2 isoform antibody

7*/822 isoform antibody

8*/901 isoform antibody

8*/941 isoform antibody

DKFZp686N23123 antibody

ER alpha antibody

ER antibody

ER-alpha antibody

Era antibody

Expand

Images

  • Application: IF-Tissue<br /><br />Species: Human<br /><br />Site: endometrium<br /><br />Sample: Paraffin-embedded section<br /><br />Antibody concentration: 1/500

    Application: IF-Tissue

    Species: Human

    Site: endometrium

    Sample: Paraffin-embedded section

    Antibody concentration: 1/500

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Estrogen Receptor alpha antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/1,000 dilution.<br /><br />Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with <a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a> for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND&reg; RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-Estrogen Receptor alpha antibody (HA721140) at 1/1,000 dilution.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. The section was incubated with HA721140 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Estrogen Receptor alpha antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/5,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Estrogen Receptor alpha antibody (HA721140) at 1/5,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721140) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Estrogen Receptor alpha antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human cervix tissue with Rabbit anti-Estrogen Receptor alpha antibody (HA721140) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Estrogen Receptor alpha antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-Estrogen Receptor alpha antibody (HA721140) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721140) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-Estrogen Receptor alpha antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721140" style="font-weight: bold;text-decoration: underline;">HA721140</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue with Rabbit anti-Estrogen Receptor alpha antibody (HA721140) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721140) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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