Uroplakin III Recombinant Rabbit Monoclonal Antibody [PD00-09]
Catalog# HA721161
Uroplakin III Recombinant Rabbit Monoclonal Antibody [PD00-09]
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IHC-P
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FC
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Human
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unconjugated
Overview
Product Name
Uroplakin III Recombinant Rabbit Monoclonal Antibody [PD00-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Uroplakin III aa 250 to the C-terminus.
Species Reactivity
Human
Validated Applications
IHC-P, FC
Target Molecular Weight
Predicted band size: 31 kDa
Positive Control
Human bladder carcinoma tissue, Hela, 293.
Conjugation
unconjugated
Clone Number
PD00-09
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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IHC-P
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1:5,000
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FC
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1:500-1:1,000
Target
Function
This gene encodes a member of the uroplakin family, a group of transmembrane proteins that form complexes on the apical surface of the bladder epithelium. Mutations in this gene may be associated with renal adysplasia. Alternatively spliced transcript variants have been described.
Background References
1. Zwaans BMM. et. al. Voiding defects in acute radiation cystitis driven by urothelial barrier defect through loss of E-cadherin, ZO-1 and Uroplakin III. Sci Rep. 2021 Sep
2. Ozturk H. et. al. Expression of NGF, MCP-1, uroplakin III, and NOS in bladder urothelium after partial urethral obstruction in rats. J Pediatr Urol. 2020 Dec
Subcellular Location
Endoplasmic reticulum membrane.
UNIPROT
Synonyms
MGC119178 antibody
OTTHUMP00000028951 antibody
UP III antibody
UP3a antibody
UPIII antibody
UPIIIA antibody
UPK 3 antibody
UPK 3A antibody
UPK3 antibody
UPK3A antibody
ExpandImages
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Immunohistochemical analysis of paraffin-embedded human bladder carcinoma tissue with Rabbit anti-Uroplakin III antibody (HA721161) at 1/5,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721161) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of Hela cells labeling Uroplakin III.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721161, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of 293 cells labeling Uroplakin III.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721161, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"