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PU.1 / Spi1 Recombinant Rabbit Monoclonal Antibody [PD00-14]

Usd: 385

Specification

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Catalog# HA721162

PU.1 / Spi1 Recombinant Rabbit Monoclonal Antibody [PD00-14]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

PU.1 / Spi1 Recombinant Rabbit Monoclonal Antibody [PD00-14]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human PU.1 aa 1-100.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Molecular Weight

Predicted band size: 31 kDa

Positive Control

Daudi cell lysate, THP-1 cell lysate, human B-cell lymphoma tissue.

Conjugation

unconjugated

Clone Number

PD00-14

RRID

AB_3072284

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Background References

1. Yashiro T. et. al. PU.1 and IRF8 Modulate Activation of NLRP3 Inflammasome via Regulating Its Expression in Human Macrophages. Front Immunol. 2021 Apr

2. Pimenova AA. et. al. Alzheimer\'s-associated PU.1 expression levels regulate microglial inflammatory response. Neurobiol Dis. 2021 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P17947 Human

Synonyms

transcription factor spi1 antibody

31 kDa Transforming Protein antibody

31 kDa-transforming protein antibody

cb1086 antibody

Hematopoietic transcription factor PU.1 antibody

OF antibody

oncogene spi1 antibody

PU.1 antibody

SFFV virus-induced murine erythroleukemia oncogene, mouse, homolog of antibody

SFPI1 antibody

Expand

Images

  • Western blot analysis of PU.1 / Spi1 on different lysates with Rabbit anti-PU.1 / Spi1 antibody (<a href="/products/HA721162" style="font-weight: bold;text-decoration: underline;">HA721162</a>) at 1/1,000 dilution.<br /><br />Lane 1: Daudi cell lysate<br />Lane 2: THP-1 cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 31 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 2 minutes;<br />12% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721162" style="font-weight: bold;text-decoration: underline;">HA721162</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1:300,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of PU.1 / Spi1 on different lysates with Rabbit anti-PU.1 / Spi1 antibody (HA721162) at 1/1,000 dilution.

    Lane 1: Daudi cell lysate
    Lane 2: THP-1 cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 31 kDa
    Observed band size: 40 kDa

    Exposure time: 2 minutes;
    12% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721162) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-PU.1 / Spi1 antibody (<a href="/products/HA721162" style="font-weight: bold;text-decoration: underline;">HA721162</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721162" style="font-weight: bold;text-decoration: underline;">HA721162</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Rabbit anti-PU.1 / Spi1 antibody (HA721162) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721162) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"